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Titel: Tracking matricellular protein SPARC in extracellular vesicles as a non-destructive method to evaluate lipid-based antifibrotic treatments
VerfasserIn: Zivko, Cristina
Fuhrmann, Kathrin
Fuhrmann, Gregor
Luciani, Paola
Sprache: Englisch
Titel: Communications biology
Bandnummer: 5
Heft: 1
Verlag/Plattform: Springer Nature
Erscheinungsjahr: 2022
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Uncovering the complex cellular mechanisms underlying hepatic fibrogenesis could expedite the development of effective treatments and noninvasive diagnosis for liver fibrosis. The biochemical complexity of extracellular vesicles (EVs) and their role in intercellular communication make them an attractive tool to look for biomarkers as potential alternative to liver biopsies. We developed a solid set of methods to isolate and characterize EVs from differently treated human hepatic stellate cell (HSC) line LX-2, and we investigated their biological effect onto naïve LX-2, proving that EVs do play an active role in fibrogenesis. We mined our proteomic data for EV-associated proteins whose expression correlated with HSC treatment, choosing the matricellular protein SPARC as proof-of-concept for the feasibility of fluorescence nanoparticle-tracking analysis to determine an EV-based HSCs' fibrogenic phenotype. We thus used EVs to directly evaluate the efficacy of treatment with S80, a polyenylphosphatidylcholines-rich lipid, finding that S80 reduces the relative presence of SPARC-positive EVs. Here we correlated the cellular response to lipid-based antifibrotic treatment to the relative presence of a candidate protein marker associated with the released EVs. Along with providing insights into polyenylphosphatidylcholines treatments, our findings pave the way for precise and less invasive diagnostic analyses of hepatic fibrogenesis.
DOI der Erstveröffentlichung: 10.1038/s42003-022-04123-z
URL der Erstveröffentlichung: https://www.nature.com/articles/s42003-022-04123-z
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-446130
hdl:20.500.11880/39772
http://dx.doi.org/10.22028/D291-44613
ISSN: 2399-3642
Datum des Eintrags: 11-Mär-2025
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Pharmazie
Professur: NT - Jun.-Prof. Dr. Gregor Fuhrmann
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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