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doi:10.22028/D291-44596
Titel: | Scalable expansion of iPSC and their derivatives across multiple lineages |
VerfasserIn: | Kwok, Chee Keong Sébastien, Isabelle Hariharan, Krithika Meiser, Ina Wihan, Jeanette Altmaier, Saskia Karnatz, Isabell Bauer, Dominic Fischer, Benjamin Feile, Alexander Cabrera-Socorro, Alfredo Rasmussen, Mikkel Holst, Bjørn Neubauer, Julia C. Clausen, Christian Verfaillie, Catherine Ebneth, Andreas Hansson, Mattias Steeg, Rachel Zimmermann, Heiko |
Sprache: | Englisch |
Titel: | Reproductive toxicology |
Bandnummer: | 112 |
Verlag/Plattform: | Elsevier |
Erscheinungsjahr: | 2022 |
DDC-Sachgruppe: | 570 Biowissenschaften, Biologie |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Induced pluripotent stem cell (iPSC) technology enabled the production of pluripotent stem cell lines from somatic cells from a range of known genetic backgrounds. Their ability to differentiate and generate a wide variety of cell types has resulted in their use for various biomedical applications, including toxicity testing. Many of these iPSC lines are now registered in databases and stored in biobanks such as the European Bank for induced pluripotent Stem Cells (EBiSC), which can streamline the quality control and distribution of these individual lines. To generate the quantities of cells for banking and applications like high-throughput toxicity screening, scalable and robust methods need to be developed to enable the large-scale production of iPSCs. 3D suspension culture platforms are increasingly being used by stem cell researchers, owing to a higher cell output in a smaller footprint, as well as simpler scaling by increasing culture volume. Here we describe our strategies for successful scalable production of iPSCs using a benchtop bioreactor and incubator for 3D suspension cultures, while maintaining quality attributes expected of high-quality iPSC lines. Additionally, to meet the increasing demand for "ready-to-use" cell types, we report recent work to establish robust, scalable differentiation protocols to cardiac, neural, and hepatic fate to enable EBiSC to increase available research tools. |
DOI der Erstveröffentlichung: | 10.1016/j.reprotox.2022.05.007 |
URL der Erstveröffentlichung: | https://www.sciencedirect.com/science/article/pii/S0890623822000685 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-445967 hdl:20.500.11880/39771 http://dx.doi.org/10.22028/D291-44596 |
ISSN: | 1873-1708 0890-6238 |
Datum des Eintrags: | 11-Mär-2025 |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Biowissenschaften |
Professur: | NT - Prof. Dr. Heiko Zimmermann |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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1-s2.0-S0890623822000685-main.pdf | 7,7 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons