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Titel: Scalable expansion of iPSC and their derivatives across multiple lineages
VerfasserIn: Kwok, Chee Keong
Sébastien, Isabelle
Hariharan, Krithika
Meiser, Ina
Wihan, Jeanette
Altmaier, Saskia
Karnatz, Isabell
Bauer, Dominic
Fischer, Benjamin
Feile, Alexander
Cabrera-Socorro, Alfredo
Rasmussen, Mikkel
Holst, Bjørn
Neubauer, Julia C.
Clausen, Christian
Verfaillie, Catherine
Ebneth, Andreas
Hansson, Mattias
Steeg, Rachel
Zimmermann, Heiko
Sprache: Englisch
Titel: Reproductive toxicology
Bandnummer: 112
Verlag/Plattform: Elsevier
Erscheinungsjahr: 2022
DDC-Sachgruppe: 570 Biowissenschaften, Biologie
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Induced pluripotent stem cell (iPSC) technology enabled the production of pluripotent stem cell lines from somatic cells from a range of known genetic backgrounds. Their ability to differentiate and generate a wide variety of cell types has resulted in their use for various biomedical applications, including toxicity testing. Many of these iPSC lines are now registered in databases and stored in biobanks such as the European Bank for induced pluripotent Stem Cells (EBiSC), which can streamline the quality control and distribution of these individual lines. To generate the quantities of cells for banking and applications like high-throughput toxicity screening, scalable and robust methods need to be developed to enable the large-scale production of iPSCs. 3D suspension culture platforms are increasingly being used by stem cell researchers, owing to a higher cell output in a smaller footprint, as well as simpler scaling by increasing culture volume. Here we describe our strategies for successful scalable production of iPSCs using a benchtop bioreactor and incubator for 3D suspension cultures, while maintaining quality attributes expected of high-quality iPSC lines. Additionally, to meet the increasing demand for "ready-to-use" cell types, we report recent work to establish robust, scalable differentiation protocols to cardiac, neural, and hepatic fate to enable EBiSC to increase available research tools.
DOI der Erstveröffentlichung: 10.1016/j.reprotox.2022.05.007
URL der Erstveröffentlichung: https://www.sciencedirect.com/science/article/pii/S0890623822000685
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-445967
hdl:20.500.11880/39771
http://dx.doi.org/10.22028/D291-44596
ISSN: 1873-1708
0890-6238
Datum des Eintrags: 11-Mär-2025
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Prof. Dr. Heiko Zimmermann
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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