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Titel: Extracellular vimentin is expressed at the rear of activated macrophage-like cells: Potential role in enhancement of migration and phagocytosis
VerfasserIn: Thalla, Divyendu Goud UdsID
Rajwar, Ashish Chand
Laurent, Annalena Maria
Becher, Johanna Elisabeth
Kainka, Lucina
Lautenschläger, Franziska UdsID
Sprache: Englisch
In:
Titel: Frontiers in cell and developmental biology
Bandnummer: 10
Verlag/Plattform: Frontiers
Erscheinungsjahr: 2022
DDC-Sachgruppe: 530 Physik
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Macrophages have a vital role in the immune system through elimination of cell debris and microorganisms by phagocytosis. The activation of macrophages by tumour necrosis factor-α induces expression of extracellular cell-surface vimentin and promotes release of this vimentin into the extracellular environment. Vimentin is a cytoskeletal protein that is primarily located in the cytoplasm of cells. However, under circumstances like injury, stress, senescence and activation, vimentin can be expressed on the extracellular cell surface, or it can be released into the extracellular space. The characteristics of this extracellular vimentin, and its implications for the functional role of macrophages and the mechanism of secretion remain unclear. Here, we demonstrate that vimentin is released mainly from the back of macrophage-like cells. This polarisation is strongly enhanced upon macrophage activation. One-dimensional patterned lines showed that extracellular cell-surface vimentin is localised primarily at the back of activated macrophage-like cells. Through two-dimensional migration and phagocytosis assays, we show that this extracellular vimentin enhances migration and phagocytosis of macrophage-like cells. We further show that this extracellular vimentin forms agglomerates on the cell surface, in contrast to its intracellular filamentous form, and that it is released into the extracellular space in the form of small fragments. Taken together, we provide new insights into the release of extracellular cell-surface vimentin and its implications for macrophage functionality.
DOI der Erstveröffentlichung: 10.3389/fcell.2022.891281
URL der Erstveröffentlichung: https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2022.891281/full
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-445500
hdl:20.500.11880/39755
http://dx.doi.org/10.22028/D291-44550
ISSN: 2296-634X
Datum des Eintrags: 5-Mär-2025
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Physik
Professur: NT - Jun.-Prof. Dr. Franziska Lautenschläger
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes



Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons