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Titel: The Shape of Human Red Blood Cells Suspended in Autologous Plasma and Serum
VerfasserIn: Fischer, Thomas M.
Sprache: Englisch
Titel: Cells : open access journal
Bandnummer: 11
Heft: 12
Verlag/Plattform: MDPI
Erscheinungsjahr: 2022
DDC-Sachgruppe: 530 Physik
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: (1) Background: In almost all studies of the shape of the human red blood cell (RBC), the suspending medium was a salt solution supplemented with albumin. However, the ratio of thickness across the dimple region to the thickness of the rim (THR) depends on the albumin concentration. Values of the THR in the literature range from 0.27 to 0.627 whereas in the present work it was 0.550 or 0.601 whether measured in plasma or serum. (2) Methods: 9911 RBCs of eight donors were suspended in autologous plasma or serum. Sedimented RBCs were observed under bright field illumination at 416 nm. From the profiles of gray value, the THR was determined. (3) Results: The THR displays a wide distribution within a single blood sample. A direct correlation of THR and spontaneous curvature of the membrane is likely. The variation of the mean THR between different donors is large. The aspect ratio of RBCs viewed face-on ranged on average from 1 to 1.48. In oval RBCs, the rim is thicker along the major axis than along the minor axis, an effect increasing with increasing aspect ratio. Remodeling of the membrane skeleton occurs in vivo with a characteristic time (τ) on the order of 1 h. (4) Conclusions: Consideration of these data in models of RBC behavior might improve the agreement with observations. τ≈1 h suggests a more general type of reference configuration of the membrane skeleton than a stress free shape.
DOI der Erstveröffentlichung: 10.3390/cells11121941
URL der Erstveröffentlichung: https://www.mdpi.com/2073-4409/11/12/1941
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-445036
hdl:20.500.11880/39741
http://dx.doi.org/10.22028/D291-44503
ISSN: 2073-4409
Datum des Eintrags: 27-Feb-2025
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Physik
Professur: NT - Keiner Professur zugeordnet
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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