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doi:10.22028/D291-44364
Titel: | Early Synapse-Specific Alterations of Photoreceptor Mitochondria in the EAE Mouse Model of Multiple Sclerosis |
VerfasserIn: | Ibrahim, Dalia R. Schwarz, Karin Suiwal, Shweta Maragkou, Sofia Schmitz, Frank |
Sprache: | Englisch |
Titel: | Cells |
Bandnummer: | 14 |
Heft: | 3 |
Verlag/Plattform: | MDPI |
Erscheinungsjahr: | 2025 |
Freie Schlagwörter: | retina ribbon synapse multiple sclerosis experimental autoimmune encephalomyelitis (EAE) mitochondria MIC60 DRP1 synaptic mitochondria extra-synaptic mitochondria |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Multiple sclerosis (MS) is an inflammatory autoimmune disease of the central nervous system (CNS) linked to many neurological disabilities. The visual system is frequently impaired in MS. In previous studies, we observed early malfunctions of rod photoreceptor ribbon synapses in the EAE mouse model of MS that included alterations in synaptic vesicle cycling and disturbances of presynaptic Ca2+ homeostasis. Since these presynaptic events are highly energy-demanding, we analyzed whether synaptic mitochondria, which play a major role in synaptic energy metabolism, might be involved at that early stage. Rod photoreceptor presynaptic terminals contain a single large mitochondrion next to the synaptic ribbon. In the present study, we analyzed the expression of functionally relevant mitochondrial proteins (MIC60, ATP5B, COX1, PINK1, DRP1) by high-resolution qualitative and quantitative immunofluorescence microscopy, immunogold electron microscopy and quantitative Western blot experiments. We observed a decreased expression of many functionally relevant proteins in the synaptic mitochondria of EAE photoreceptors at an early stage, suggesting that early mitochondrial dysfunctions play an important role in the early synapse pathology. Interestingly, mitochondria in presynaptic photoreceptor terminals were strongly compromised in early EAE, whereas extra-synaptic mitochondria in photoreceptor inner segments remained unchanged, demonstrating a functional heterogeneity of photoreceptor mitochondria. |
DOI der Erstveröffentlichung: | 10.3390/cells14030206 |
URL der Erstveröffentlichung: | https://doi.org/10.3390/cells14030206 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-443648 hdl:20.500.11880/39691 http://dx.doi.org/10.22028/D291-44364 |
ISSN: | 2073-4409 |
Datum des Eintrags: | 19-Feb-2025 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Anatomie und Zellbiologie |
Professur: | M - Prof. Dr. Frank Schmitz |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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cells-14-00206-v2.pdf | 14,96 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons