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doi:10.22028/D291-44348
Titel: | Transcriptomics of a KDELR1 knockout cell line reveals modulated cell adhesion properties |
VerfasserIn: | Blum, Andrea Khalifa, Saleem Nordström, Karl Simon, Martin Schulz, Marcel H. Schmitt, Manfred J. |
Sprache: | Englisch |
Titel: | Scientific reports |
Bandnummer: | 9 |
Heft: | 1 |
Verlag/Plattform: | Springer Nature |
Erscheinungsjahr: | 2019 |
DDC-Sachgruppe: | 570 Biowissenschaften, Biologie |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | KDEL receptors (KDELRs) represent transmembrane proteins of the secretory pathway which regulate the retention of soluble ER-residents as well as retrograde and anterograde vesicle trafficking. In addition, KDELRs are involved in the regulation of cellular stress response and ECM degradation. For a deeper insight into KDELR1 specific functions, we characterised a KDELR1-KO cell line (HAP1) through whole transcriptome analysis by comparing KDELR1-KO cells with its respective HAP1 wild-type. Our data indicate more than 300 significantly and differentially expressed genes whose gene products are mainly involved in developmental processes such as cell adhesion and ECM composition, pointing out to severe cellular disorders due to a loss of KDELR1. Impaired adhesion capacity of KDELR1-KO cells was further demonstrated through in vitro adhesion assays, while collagen- and/or laminin-coating nearly doubled the adhesion property of KDELR1-KO cells compared to wild-type, confirming a transcriptional adaptation to improve or restore the cellular adhesion capability. Perturbations within the secretory pathway were verified by an increased secretion of ER-resident PDI and decreased cell viability under ER stress conditions, suggesting KDELR1-KO cells to be severely impaired in maintaining cellular homeostasis. |
DOI der Erstveröffentlichung: | 10.1038/s41598-019-47027-5 |
URL der Erstveröffentlichung: | https://www.nature.com/articles/s41598-019-47027-5 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-443485 hdl:20.500.11880/39638 http://dx.doi.org/10.22028/D291-44348 |
ISSN: | 2045-2322 |
Datum des Eintrags: | 12-Feb-2025 |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Biowissenschaften |
Professur: | NT - Keiner Professur zugeordnet |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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s41598-019-47027-5.pdf | 1,2 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons