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Titel: Transcriptomics of a KDELR1 knockout cell line reveals modulated cell adhesion properties
VerfasserIn: Blum, Andrea
Khalifa, Saleem
Nordström, Karl
Simon, Martin
Schulz, Marcel H.
Schmitt, Manfred J.
Sprache: Englisch
Titel: Scientific reports
Bandnummer: 9
Heft: 1
Verlag/Plattform: Springer Nature
Erscheinungsjahr: 2019
DDC-Sachgruppe: 570 Biowissenschaften, Biologie
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: KDEL receptors (KDELRs) represent transmembrane proteins of the secretory pathway which regulate the retention of soluble ER-residents as well as retrograde and anterograde vesicle trafficking. In addition, KDELRs are involved in the regulation of cellular stress response and ECM degradation. For a deeper insight into KDELR1 specific functions, we characterised a KDELR1-KO cell line (HAP1) through whole transcriptome analysis by comparing KDELR1-KO cells with its respective HAP1 wild-type. Our data indicate more than 300 significantly and differentially expressed genes whose gene products are mainly involved in developmental processes such as cell adhesion and ECM composition, pointing out to severe cellular disorders due to a loss of KDELR1. Impaired adhesion capacity of KDELR1-KO cells was further demonstrated through in vitro adhesion assays, while collagen- and/or laminin-coating nearly doubled the adhesion property of KDELR1-KO cells compared to wild-type, confirming a transcriptional adaptation to improve or restore the cellular adhesion capability. Perturbations within the secretory pathway were verified by an increased secretion of ER-resident PDI and decreased cell viability under ER stress conditions, suggesting KDELR1-KO cells to be severely impaired in maintaining cellular homeostasis.
DOI der Erstveröffentlichung: 10.1038/s41598-019-47027-5
URL der Erstveröffentlichung: https://www.nature.com/articles/s41598-019-47027-5
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-443485
hdl:20.500.11880/39638
http://dx.doi.org/10.22028/D291-44348
ISSN: 2045-2322
Datum des Eintrags: 12-Feb-2025
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Keiner Professur zugeordnet
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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