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Titel: Review—Quantification of Hydrogen Peroxide by Electrochemical Methods and Electron Spin Resonance Spectroscopy
VerfasserIn: Gulaboski, Rubin
Mirčeski, Valentin
Kappl, Reinhard
Hoth, Markus
Bozem, Monika
Sprache: Englisch
Titel: Journal of the Electrochemical Society : JES
Bandnummer: 166
Heft: 8
Verlag/Plattform: IOP Publishing
Erscheinungsjahr: 2019
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Reactive oxygen species (ROS) exhibit different spatial and temporal distributions as well as concentrations in- and outside the cell, thereby functioning as signaling or pathogen-destroying molecules. Especially the ROS H2O2 is important for the patho/physiological status of an organism. Electrochemistry (EM) and electron spin resonance (ESR)-based techniques allow quantification of H2O2 in artificial and living systems, coping a concentration range from low nM up to mM. Working electrodes for EM are optimized by diverse modifications and, additionally, redox mediators are used. Ultramicroelectrodes allow scanning of single cells to spatially resolve and quantify extracellular H2O2 in real-time. With ESR spectroscopy, •O2¯, but not H2O2, can be directly determined by spin probes in- and outside of cells in suspensions. Monitoring H2O2 requires formation of intermediate radicals, detectable with spin probes. Low μM [H2O2] can thus be assessed specifically. Using suitable spin traps, in-vivo ESR and immuno-spin trapping can visualize different radicals at their respective production sites in small animals, organs and tissues. Here, the redox reaction cascades may interfere with cell metabolism. Optimization of all methods established for H2O2 determination would be favorable to finally combine them for mutual validation. Thus, a deeper insight into cellular ROS metabolism can be obtained.
DOI der Erstveröffentlichung: 10.1149/2.1061908jes
URL der Erstveröffentlichung: https://iopscience.iop.org/article/10.1149/2.1061908jes
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-442967
hdl:20.500.11880/39619
http://dx.doi.org/10.22028/D291-44296
ISSN: 1945-7111
0013-4651
Datum des Eintrags: 10-Feb-2025
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Biophysik
Professur: M - Prof. Dr. Markus Hoth
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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