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Titel: Enoxaparin does not affect network formation of adipose tissue-derived microvascular fragments
VerfasserIn: Später, Thomas
Frueh, Florian S.
Karschnia, Philipp
Menger, Michael D.
Laschke, Matthias W.
Sprache: Englisch
Titel: Wound Repair and Regeneration
Bandnummer: 26
Heft: 1
Seiten: 36-45
Verlag/Plattform: Wiley
Erscheinungsjahr: 2018
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Dermal substitutes are frequently used for the initial coverage of extensive skin defects. The seeding of these implants with adipose tissue–derived microvascular fragments (ad-MVF) has recently been shown to accelerate their vascularization and incorporation. In the present study we analyzed whether these processes are affected by a thromboprophylactic therapy with the low molecular weight heparin (LMWH) enoxaparin (enox). Green fluorescent protein (GFP)1 ad-MVF were isolated from enox- (8 mg/kg s.c.) and vehicle-treated (0.9% NaCl s.c.) (C57BL/6-Tg(CAG-EGFP)1Osb/J mice and seeded onto Integra matrices. Subsequently, these were implanted into full-thickness skin defects within dorsal skinfold chambers of enox- and vehicle-treated C57BL/6 wild-type mice. Repetitive stereomicroscopy and intravital fluorescence microscopy over 2 weeks as well as histological and immunohistochemical analyses on day 14 revealed that enox does not inhibit the reassembly of ad-MVF into new microvascular networks. In addition, treatment with the anticoagulative compound did not promote implant-induced hemorrhage formation. Accordingly, Integra matrices in enox- and vehicle-treated animals exhibited a comparable final microvessel density, fraction of GFP1 blood vessels originating from seeded ad-MVF, collagen fiber content, and epithelialization. These novel findings demonstrate that the seeding of dermal substitutes with ad-MVF may be applied also during thromboprophylactic therapy without affecting implant vascularization and bleeding risk.
DOI der Erstveröffentlichung: 10.1111/wrr.12621
URL der Erstveröffentlichung: https://doi.org/10.1111/wrr.12621
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-442140
hdl:20.500.11880/39522
http://dx.doi.org/10.22028/D291-44214
ISSN: 1524-475X
1067-1927
Datum des Eintrags: 29-Jan-2025
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Chirurgie
Professur: M - Prof. Dr. Michael D. Menger
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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