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doi:10.22028/D291-44205
Titel: | Erythropoietin promotes network formation of transplanted adipose tissue-derived microvascular fragments |
VerfasserIn: | Karschnia, P. Scheuer, C. Heß, A. Später, T. Menger, M. D. Laschke, M. W. |
Sprache: | Englisch |
Titel: | European Cells and Materials |
Bandnummer: | 35 |
Seiten: | 268-280 |
Verlag/Plattform: | University of Wales Press |
Erscheinungsjahr: | 2018 |
Freie Schlagwörter: | Tissue-engineering erythropoietin microvascular fragments angiogenesis vascularisation scaffold apoptosis proliferation |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | The seeding of tissue constructs with adipose tissue-derived microvascular fragments (ad-MVF) is an emerging pre-vascularisation strategy. Ad-MVF rapidly reassemble into new microvascular networks after in vivo implantation. Herein it was analysed whether this process was improved by erythropoietin (EPO). Ad-MVF were isolated from green fluorescent protein (GFP)+ as well as wild-type C57BL/6 mice and cultivated for 24 h in medium supplemented with EPO (20 IU/mL) or vehicle. Freshly isolated, non-cultivated ad-MVF served as controls. Protein expression, cell viability and proliferation of ad-MVF were assessed by proteome profiler array and fluorescence microscopy. GFP+ ad-MVF were seeded on collagen-glycosaminoglycan matrices, which were implanted into dorsal skinfold chambers of C57BL/6 mice, to analyse their vascularisation over 14 d by intravital fluorescence microscopy, histology and immunohistochemistry. Cultivation up-regulated the expression of pro- and anti-angiogenic factors within both vehicle- and EPO-treated ad-MVF when compared with non-cultivated controls. Moreover, EPO treatment suppressed cultivation-associated apoptosis and significantly increased the number of proliferating endothelial cells in ad-MVF when compared with vehicletreated and non-cultivated ad-MVF. Accordingly, implanted matrices seeded with EPO-treated ad-MVF exhibited an improved vascularisation, as indicated by a significantly higher functional microvessel density. The matrices of the three groups contained a comparably large fraction of GFP+ microvessels originating from the ad-MVF, whereas the tissue surrounding the matrices seeded with EPO-treated ad-MVF exhibited a significantly increased microvessel density when compared with the other two groups. These findings indicated that EPO represents a promising cytokine to further boost the excellent vascularisation properties of ad-MVF in tissue-engineering applications. |
DOI der Erstveröffentlichung: | 10.22203/eCM.v035a19 |
URL der Erstveröffentlichung: | https://doi.org/10.22203/ecm.v035a19 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-442054 hdl:20.500.11880/39518 http://dx.doi.org/10.22028/D291-44205 |
ISSN: | 1473-2262 |
Datum des Eintrags: | 29-Jan-2025 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Chirurgie |
Professur: | M - Prof. Dr. Michael D. Menger |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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