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Titel: Erythropoietin promotes network formation of transplanted adipose tissue-derived microvascular fragments
VerfasserIn: Karschnia, P.
Scheuer, C.
Heß, A.
Später, T.
Menger, M. D.
Laschke, M. W.
Sprache: Englisch
Titel: European Cells and Materials
Bandnummer: 35
Seiten: 268-280
Verlag/Plattform: University of Wales Press
Erscheinungsjahr: 2018
Freie Schlagwörter: Tissue-engineering
erythropoietin
microvascular fragments
angiogenesis
vascularisation
scaffold
apoptosis
proliferation
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: The seeding of tissue constructs with adipose tissue-derived microvascular fragments (ad-MVF) is an emerging pre-vascularisation strategy. Ad-MVF rapidly reassemble into new microvascular networks after in vivo implantation. Herein it was analysed whether this process was improved by erythropoietin (EPO). Ad-MVF were isolated from green fluorescent protein (GFP)+ as well as wild-type C57BL/6 mice and cultivated for 24 h in medium supplemented with EPO (20 IU/mL) or vehicle. Freshly isolated, non-cultivated ad-MVF served as controls. Protein expression, cell viability and proliferation of ad-MVF were assessed by proteome profiler array and fluorescence microscopy. GFP+ ad-MVF were seeded on collagen-glycosaminoglycan matrices, which were implanted into dorsal skinfold chambers of C57BL/6 mice, to analyse their vascularisation over 14 d by intravital fluorescence microscopy, histology and immunohistochemistry. Cultivation up-regulated the expression of pro- and anti-angiogenic factors within both vehicle- and EPO-treated ad-MVF when compared with non-cultivated controls. Moreover, EPO treatment suppressed cultivation-associated apoptosis and significantly increased the number of proliferating endothelial cells in ad-MVF when compared with vehicletreated and non-cultivated ad-MVF. Accordingly, implanted matrices seeded with EPO-treated ad-MVF exhibited an improved vascularisation, as indicated by a significantly higher functional microvessel density. The matrices of the three groups contained a comparably large fraction of GFP+ microvessels originating from the ad-MVF, whereas the tissue surrounding the matrices seeded with EPO-treated ad-MVF exhibited a significantly increased microvessel density when compared with the other two groups. These findings indicated that EPO represents a promising cytokine to further boost the excellent vascularisation properties of ad-MVF in tissue-engineering applications.
DOI der Erstveröffentlichung: 10.22203/eCM.v035a19
URL der Erstveröffentlichung: https://doi.org/10.22203/ecm.v035a19
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-442054
hdl:20.500.11880/39518
http://dx.doi.org/10.22028/D291-44205
ISSN: 1473-2262
Datum des Eintrags: 29-Jan-2025
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Chirurgie
Professur: M - Prof. Dr. Michael D. Menger
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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