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doi:10.22028/D291-44202
Titel: | Prevascularization of dermal substitutes with adipose tissue-derived microvascular fragments enhances early skin grafting |
VerfasserIn: | Frueh, Florian S. Später, Thomas Körbel, Christina Scheuer, Claudia Simson, Anna C. Lindenblatt, Nicole Giovanoli, Pietro Menger, Michael D. Laschke, Matthias W. |
Sprache: | Englisch |
Titel: | Scientific Reports |
Bandnummer: | 8 |
Heft: | 1 |
Verlag/Plattform: | Springer Nature |
Erscheinungsjahr: | 2018 |
Freie Schlagwörter: | Preclinical research Tissue engineering |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Split-thickness skin grafts (STSG) are still the gold standard for the treatment of most skin defects. Hence, there is an ongoing need to improve this procedure. For this purpose, we herein analyzed dermal matrices seeded with adipose tissue-derived microvascular fragments (ad-MVF) in a bradythrophic wound model. In additional experiments, the matrices were covered with autologous STSG 10 days after implantation. Green fuorescence protein (GFP)+ ad-MVF were isolated from C57BL/6- Tg(CAG-EGFP)1Osb/J mice and seeded onto collagen-glycosaminoglycan matrices. Non-seeded and prevascularized matrices were implanted into full-thickness skin defects on the skull of CD1 nu/nu mice for 21 days. Vascularization, lymphangiogenesis and incorporation of the matrices were analyzed using photo-acoustic imaging, trans-illumination stereomicroscopy, histology, and immunohistochemistry. The survival rate of STSG was assessed by planimetry. After 21 days, the density of microvascular and lymphatic networks was signifcantly higher in prevascularized matrices when compared to controls. This was associated with an improved implant integration. Moreover, prevascularization with ad-MVF allowed successful autologous skin grafting already at day 10, while coverage of non-seeded controls at day 10 resulted in STSG necrosis. In conclusion, ad-MVF represent powerful vascularization units. Seeded on dermal substitutes, they accelerate and enhance the healing of full-thickness skin defects and allow early coverage with STSG. |
DOI der Erstveröffentlichung: | 10.1038/s41598-018-29252-6 |
URL der Erstveröffentlichung: | https://www.nature.com/articles/s41598-018-29252-6 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-442029 hdl:20.500.11880/39516 http://dx.doi.org/10.22028/D291-44202 |
ISSN: | 2045-2322 |
Datum des Eintrags: | 29-Jan-2025 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Chirurgie |
Professur: | M - Prof. Dr. Michael D. Menger |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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s41598-018-29252-6.pdf | 1,82 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons