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Titel: Prevascularization of dermal substitutes with adipose tissue-derived microvascular fragments enhances early skin grafting
VerfasserIn: Frueh, Florian S.
Später, Thomas
Körbel, Christina
Scheuer, Claudia
Simson, Anna C.
Lindenblatt, Nicole
Giovanoli, Pietro
Menger, Michael D.
Laschke, Matthias W.
Sprache: Englisch
Titel: Scientific Reports
Bandnummer: 8
Heft: 1
Verlag/Plattform: Springer Nature
Erscheinungsjahr: 2018
Freie Schlagwörter: Preclinical research
Tissue engineering
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Split-thickness skin grafts (STSG) are still the gold standard for the treatment of most skin defects. Hence, there is an ongoing need to improve this procedure. For this purpose, we herein analyzed dermal matrices seeded with adipose tissue-derived microvascular fragments (ad-MVF) in a bradythrophic wound model. In additional experiments, the matrices were covered with autologous STSG 10 days after implantation. Green fuorescence protein (GFP)+ ad-MVF were isolated from C57BL/6- Tg(CAG-EGFP)1Osb/J mice and seeded onto collagen-glycosaminoglycan matrices. Non-seeded and prevascularized matrices were implanted into full-thickness skin defects on the skull of CD1 nu/nu mice for 21 days. Vascularization, lymphangiogenesis and incorporation of the matrices were analyzed using photo-acoustic imaging, trans-illumination stereomicroscopy, histology, and immunohistochemistry. The survival rate of STSG was assessed by planimetry. After 21 days, the density of microvascular and lymphatic networks was signifcantly higher in prevascularized matrices when compared to controls. This was associated with an improved implant integration. Moreover, prevascularization with ad-MVF allowed successful autologous skin grafting already at day 10, while coverage of non-seeded controls at day 10 resulted in STSG necrosis. In conclusion, ad-MVF represent powerful vascularization units. Seeded on dermal substitutes, they accelerate and enhance the healing of full-thickness skin defects and allow early coverage with STSG.
DOI der Erstveröffentlichung: 10.1038/s41598-018-29252-6
URL der Erstveröffentlichung: https://www.nature.com/articles/s41598-018-29252-6
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-442029
hdl:20.500.11880/39516
http://dx.doi.org/10.22028/D291-44202
ISSN: 2045-2322
Datum des Eintrags: 29-Jan-2025
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Chirurgie
Professur: M - Prof. Dr. Michael D. Menger
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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