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doi:10.22028/D291-44201
Titel: | Effects of locally applied adipose tissue-derived microvascular fragments by thermoresponsive hydrogel on bone healing |
VerfasserIn: | Orth, M. Altmeyer, M. A. B. Scheuer, C. Braun, B. J. Holstein, J. H. Eglin, D. D'Este, M. Histing, T. Laschke, M. W. Pohlemann, T. Menger, M. D. |
Sprache: | Englisch |
Titel: | Acta Biomaterialia |
Bandnummer: | 77 |
Seiten: | 201-211 |
Verlag/Plattform: | Elsevier |
Erscheinungsjahr: | 2018 |
Freie Schlagwörter: | Microvascular fragments Thermoresponsive hydrogel Bone healing Vascularization VEGF |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Insufficient vascularization is a major cause for the development of non-unions. To overcome this problem, adipose tissue-derived microvascular fragments (MVF) may serve as vascularization units. However, their application into bone defects needs a carrier system. Herein, we analyzed whether this is achieved by a thermoresponsive hydrogel (TRH). MVF were isolated from CD-1 mice and cultivated after incorporation into TRH, while non-incorporated MVF served as controls. Viability of MVF was assessed immunohistochemically over a 7-day period. Moreover, osteotomies were induced in femurs of CD-1 mice. The osteotomy gaps were filled with MVF-loaded TRH (TRH + MVF), unloaded TRH (TRH) or no material (control). Bone healing was evaluated 14 and 35 days postoperatively. MVF incorporated into TRH exhibited less apoptotic cells and showed a stable vessel morphology compared to controls. Micro-computed tomography revealed a reduced bone volume in TRH + MVF femurs. Histomorphometry showed less bone and more fibrous tissue after 35 days in TRH + MVF femurs compared to controls. Accordingly, TRH + MVF femurs exhibited a lower osseous bridging score and a reduced bending stiffness. Histology and Western blot analysis revealed an increased vascularization and CD31 expression, whereas vascular endothelial growth factor (VEGF) expression was reduced in TRH + MVF femurs. Furthermore, the callus of TRH + MVF femurs showed increased receptor activator of NF-jB ligand expression and higher numbers of osteoclasts. These findings indicate that TRH is an appropriate carrier system for MVF. Application of TRH + MVF increases the vascularization of bone defects. However, this impairs bone healing, most likely due to lower VEGF expression during the early course of bone healing. |
DOI der Erstveröffentlichung: | 10.1016/j.actbio.2018.07.029 |
URL der Erstveröffentlichung: | https://doi.org/10.1016/j.actbio.2018.07.029 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-442014 hdl:20.500.11880/39515 http://dx.doi.org/10.22028/D291-44201 |
ISSN: | 1742-7061 |
Datum des Eintrags: | 29-Jan-2025 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Chirurgie |
Professur: | M - Prof. Dr. Michael D. Menger M - Prof. Dr. Tim Pohlemann |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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