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Titel: Effects of locally applied adipose tissue-derived microvascular fragments by thermoresponsive hydrogel on bone healing
VerfasserIn: Orth, M.
Altmeyer, M. A. B.
Scheuer, C.
Braun, B. J.
Holstein, J. H.
Eglin, D.
D'Este, M.
Histing, T.
Laschke, M. W.
Pohlemann, T.
Menger, M. D.
Sprache: Englisch
Titel: Acta Biomaterialia
Bandnummer: 77
Seiten: 201-211
Verlag/Plattform: Elsevier
Erscheinungsjahr: 2018
Freie Schlagwörter: Microvascular fragments
Thermoresponsive hydrogel
Bone healing
Vascularization
VEGF
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Insufficient vascularization is a major cause for the development of non-unions. To overcome this problem, adipose tissue-derived microvascular fragments (MVF) may serve as vascularization units. However, their application into bone defects needs a carrier system. Herein, we analyzed whether this is achieved by a thermoresponsive hydrogel (TRH). MVF were isolated from CD-1 mice and cultivated after incorporation into TRH, while non-incorporated MVF served as controls. Viability of MVF was assessed immunohistochemically over a 7-day period. Moreover, osteotomies were induced in femurs of CD-1 mice. The osteotomy gaps were filled with MVF-loaded TRH (TRH + MVF), unloaded TRH (TRH) or no material (control). Bone healing was evaluated 14 and 35 days postoperatively. MVF incorporated into TRH exhibited less apoptotic cells and showed a stable vessel morphology compared to controls. Micro-computed tomography revealed a reduced bone volume in TRH + MVF femurs. Histomorphometry showed less bone and more fibrous tissue after 35 days in TRH + MVF femurs compared to controls. Accordingly, TRH + MVF femurs exhibited a lower osseous bridging score and a reduced bending stiffness. Histology and Western blot analysis revealed an increased vascularization and CD31 expression, whereas vascular endothelial growth factor (VEGF) expression was reduced in TRH + MVF femurs. Furthermore, the callus of TRH + MVF femurs showed increased receptor activator of NF-jB ligand expression and higher numbers of osteoclasts. These findings indicate that TRH is an appropriate carrier system for MVF. Application of TRH + MVF increases the vascularization of bone defects. However, this impairs bone healing, most likely due to lower VEGF expression during the early course of bone healing.
DOI der Erstveröffentlichung: 10.1016/j.actbio.2018.07.029
URL der Erstveröffentlichung: https://doi.org/10.1016/j.actbio.2018.07.029
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-442014
hdl:20.500.11880/39515
http://dx.doi.org/10.22028/D291-44201
ISSN: 1742-7061
Datum des Eintrags: 29-Jan-2025
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Chirurgie
Professur: M - Prof. Dr. Michael D. Menger
M - Prof. Dr. Tim Pohlemann
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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