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Titel: In Vitro and in Vivo Analysis of Adhesive, Anti-Inflammatory, and Proangiogenic Properties of Novel 3D Printed Hyaluronic Acid Glycidyl Methacrylate Hydrogel Scaffolds for Tissue Engineering
VerfasserIn: Später, Thomas
Mariyanats, Aleksandra O.
Syachina, Maria A.
Mironov, Anton V.
Savelyev, Alexander G.
Sochilina, Anastasia V.
Menger, Michael D.
Vishnyakova, Polina A.
Kananykhina, Evgeniya Y.
Fatkhudinov, Timur Kh.
Sukhikh, Gennady T.
Spitkovsky, Dmitry D.
Katsen-Globa, Alisa
Laschke, Matthias W.
Popov, Vladimir K.
Sprache: Englisch
Titel: ACS Biomaterials Science & Engineering
Bandnummer: 6
Heft: 10
Seiten: 5744-5757
Verlag/Plattform: ACS
Erscheinungsjahr: 2020
Freie Schlagwörter: polylactic-co-glycolic acid
hyaluronic acid
3D printing
angiogenesis
inflammation
dorsal skinfold chamber model
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: In this study, we prepared hydrogel scaffolds for tissue engineering by computer-assisted extrusion three-dimensional (3D) printing with photocured (λ = 445 nm) hyaluronic acid glycidyl methacrylate (HAGM). The developed product was compared with the polylactic-co-glycolic acid (PLGA) scaffolds generated by means of the original antisolvent 3D printing methodology. The cytotoxicity and cytocompatibility of the scaffolds were analyzed in vitro by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide tests, flow cytometry, and scanning electron microscopy. Anti-inflammatory and proangiogenic properties of the scaffolds were evaluated in the dorsal skinfold chamber mouse model by means of intravital fluorescence microscopy, histology, and immunohistochemistry throughout an observation period of 14 days. In vitro, none of the scaffolds revealed cytotoxicity on days 1, 2, and 5 after seeding with umbilical cord-derived multipotent stromal cells, and the primary cell adhesion to the surface of HAGM scaffolds was low. In vivo, implanted HAGM scaffolds showed enhanced vascularization and host tissue ingrowth, and the inflammatory response to them was less pronounced compared with PLGA scaffolds. The results indicate excellent biocompatibility and vascularization capacity of the developed 3D printed HAGM scaffolds and position them as strong candidates for advanced tissue engineering applications.
DOI der Erstveröffentlichung: 10.1021/acsbiomaterials.0c00741
URL der Erstveröffentlichung: https://pubs.acs.org/doi/10.1021/acsbiomaterials.0c00741
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-441335
hdl:20.500.11880/39465
http://dx.doi.org/10.22028/D291-44133
ISSN: 2373-9878
Datum des Eintrags: 24-Jan-2025
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Chirurgie
Professur: M - Prof. Dr. Michael D. Menger
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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