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doi:10.22028/D291-44114
Titel: | Systemic low-dose erythropoietin administration improves the vascularization of collagen-glycosaminoglycan matrices seeded with adipose tissue-derived microvascular fragments |
VerfasserIn: | Später, Thomas Worringer, Denise Ms. Menger, Maximilian M. Menger, Michael D. Laschke, Matthias W. |
Sprache: | Englisch |
Titel: | Journal of Tissue Engineering |
Bandnummer: | 12 |
Verlag/Plattform: | Sage |
Erscheinungsjahr: | 2021 |
Freie Schlagwörter: | Tissue engineering microvascular fragments vascularization erythropoietin wound healing |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Adipose tissue-derived microvascular fragments (MVF) are used as vascularization units in tissue engineering. In this study, we investigated whether the vascularization capacity of MVF can be improved by systemic low-dose erythropoietin (EPO) administration. MVF were isolated from the epididymal fat of donor mice and seeded onto collagen-glycosaminoglycan matrices, which were implanted into full-thickness skin defects within dorsal skinfold chambers of recipient mice. Both donor and recipient mice were treated daily with either EPO (500 IU/kg) or vehicle (0.9% NaCl). The implants were analyzed by stereomicroscopy, intravital fluorescence microscopy, histology, and immunohistochemistry. EPO-treated MVF contained a comparable number of proliferating Ki67+ but less apoptotic cleaved caspase-3+ endothelial cells when compared to vehicle-treated controls. Moreover, EPO treatment accelerated and improved the in vivo vascularization, blood vessel maturation, and epithelialization of MVF-seeded matrices. These findings indicate that systemic low-dose EPO treatment is suitable to enhance the viability and network-forming capacity of MVF. |
DOI der Erstveröffentlichung: | 10.1177/20417314211000304 |
URL der Erstveröffentlichung: | https://doi.org/10.1177/20417314211000304 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-441141 hdl:20.500.11880/39459 http://dx.doi.org/10.22028/D291-44114 |
ISSN: | 2041-7314 |
Datum des Eintrags: | 23-Jan-2025 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Chirurgie |
Professur: | M - Prof. Dr. Michael D. Menger M - Prof. Dr. Tim Pohlemann |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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spater-et-al-2021-systemic-low-dose-erythropoietin-administration-improves-the-vascularization-of-collagen.pdf | 3,76 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons