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Titel: Systemic low-dose erythropoietin administration improves the vascularization of collagen-glycosaminoglycan matrices seeded with adipose tissue-derived microvascular fragments
VerfasserIn: Später, Thomas
Worringer, Denise Ms.
Menger, Maximilian M.
Menger, Michael D.
Laschke, Matthias W.
Sprache: Englisch
Titel: Journal of Tissue Engineering
Bandnummer: 12
Verlag/Plattform: Sage
Erscheinungsjahr: 2021
Freie Schlagwörter: Tissue engineering
microvascular fragments
vascularization
erythropoietin
wound healing
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Adipose tissue-derived microvascular fragments (MVF) are used as vascularization units in tissue engineering. In this study, we investigated whether the vascularization capacity of MVF can be improved by systemic low-dose erythropoietin (EPO) administration. MVF were isolated from the epididymal fat of donor mice and seeded onto collagen-glycosaminoglycan matrices, which were implanted into full-thickness skin defects within dorsal skinfold chambers of recipient mice. Both donor and recipient mice were treated daily with either EPO (500 IU/kg) or vehicle (0.9% NaCl). The implants were analyzed by stereomicroscopy, intravital fluorescence microscopy, histology, and immunohistochemistry. EPO-treated MVF contained a comparable number of proliferating Ki67+ but less apoptotic cleaved caspase-3+ endothelial cells when compared to vehicle-treated controls. Moreover, EPO treatment accelerated and improved the in vivo vascularization, blood vessel maturation, and epithelialization of MVF-seeded matrices. These findings indicate that systemic low-dose EPO treatment is suitable to enhance the viability and network-forming capacity of MVF.
DOI der Erstveröffentlichung: 10.1177/20417314211000304
URL der Erstveröffentlichung: https://doi.org/10.1177/20417314211000304
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-441141
hdl:20.500.11880/39459
http://dx.doi.org/10.22028/D291-44114
ISSN: 2041-7314
Datum des Eintrags: 23-Jan-2025
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Chirurgie
Professur: M - Prof. Dr. Michael D. Menger
M - Prof. Dr. Tim Pohlemann
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes



Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons