Please use this identifier to cite or link to this item: doi:10.22028/D291-44107
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Title: Vesicle-bound regulatory RNAs are associated with tissue aging
Author(s): Kern, Fabian
Kuhn, Thomas
Ludwig, Nicole
Simon, Martin
Gröger, Laura
Fabis, Natalie
Salhab, Abdulrahman
Fehlmann, Tobias
Hahn, Oliver
Engel, Annika
Koch, Marcus
Koehler, Jana
Winek, Katarzyna
Soreq, Hermona
Fuhrmann, Gregor
Wyss-Coray, Tony
Meese, Eckart
Laschke, Matthias W.
Keller, Andreas
Language: English
Publisher/Platform: bioRxiv
Year of Publication: 2021
Free key words: non-coding RNA
tissue aging
extracellular vesicles
plasma
exosomes
DDC notations: 610 Medicine and health
Publikation type: Other
Abstract: Previous work on murine models and human demonstrated global as well as tissue-specific molecular aging trajectories in solid tissues and body fluids1–8. Extracellular vesicles like exosomes play a crucial role in communication and information exchange in between such systemic factors and solid tissues9,10. We sequenced freely circulating and vesicle-bound small regulatory RNAs in mice at five time points across the average life span from 2 to 18 months. Intriguingly, each small RNA class exhibits unique aging patterns, which showed differential signatures between vesicle-bound and freely circulating molecules. In particular, tRNA fragments showed overall highest correlation with aging which also matched well between sample types, facilitating age prediction with non-negative matrix factorization (86% accuracy). Interestingly, rRNAs exhibited inverse correlation trajectories between vesicles and plasma while vesicle-bound microRNAs (miRNAs) were exceptionally strong associated with aging. Affected miRNAs regulate the inflammatory response and transcriptional processes, and adipose tissues show considerable effects in associated gene regulatory modules. Finally, nanoparticle tracking and electron microscopy suggest a shift from overall many small to fewer but larger vesicles in aged plasma, potentially contributing to systemic aging trajectories and affecting the molecular aging of organs.
DOI of the first publication: 10.1101/2021.05.07.443093
URL of the first publication: https://doi.org/10.1101/2021.05.07.443093
Link to this record: urn:nbn:de:bsz:291--ds-441074
hdl:20.500.11880/39452
http://dx.doi.org/10.22028/D291-44107
Date of registration: 23-Jan-2025
Description of the related object: Supplementary material
Related object: https://www.biorxiv.org/content/biorxiv/early/2021/05/08/2021.05.07.443093/DC1/embed/media-1.pdf?download=true
https://www.biorxiv.org/content/biorxiv/early/2021/05/08/2021.05.07.443093/DC1/embed/media-1.pdf?download=true
https://www.biorxiv.org/content/biorxiv/early/2021/05/08/2021.05.07.443093/DC3/embed/media-3.xlsx?download=true
Notes: Preprint
Faculty: M - Medizinische Fakultät
NT - Naturwissenschaftlich- Technische Fakultät
Department: M - Chirurgie
M - Humangenetik
M - Medizinische Biometrie, Epidemiologie und medizinische Informatik
NT - Biowissenschaften
NT - Pharmazie
Professorship: M - Univ.-Prof. Dr. Andreas Keller
M - Prof. Dr. Eckart Meese
M - Prof. Dr. Michael D. Menger
NT - Jun.-Prof. Dr. Gregor Fuhrmann
NT - Prof. Dr. Jörn Walter
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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