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doi:10.22028/D291-44107
Title: | Vesicle-bound regulatory RNAs are associated with tissue aging |
Author(s): | Kern, Fabian Kuhn, Thomas Ludwig, Nicole Simon, Martin Gröger, Laura Fabis, Natalie Salhab, Abdulrahman Fehlmann, Tobias Hahn, Oliver Engel, Annika Koch, Marcus Koehler, Jana Winek, Katarzyna Soreq, Hermona Fuhrmann, Gregor Wyss-Coray, Tony Meese, Eckart Laschke, Matthias W. Keller, Andreas |
Language: | English |
Publisher/Platform: | bioRxiv |
Year of Publication: | 2021 |
Free key words: | non-coding RNA tissue aging extracellular vesicles plasma exosomes |
DDC notations: | 610 Medicine and health |
Publikation type: | Other |
Abstract: | Previous work on murine models and human demonstrated global as well as tissue-specific molecular aging trajectories in solid tissues and body fluids1–8. Extracellular vesicles like exosomes play a crucial role in communication and information exchange in between such systemic factors and solid tissues9,10. We sequenced freely circulating and vesicle-bound small regulatory RNAs in mice at five time points across the average life span from 2 to 18 months. Intriguingly, each small RNA class exhibits unique aging patterns, which showed differential signatures between vesicle-bound and freely circulating molecules. In particular, tRNA fragments showed overall highest correlation with aging which also matched well between sample types, facilitating age prediction with non-negative matrix factorization (86% accuracy). Interestingly, rRNAs exhibited inverse correlation trajectories between vesicles and plasma while vesicle-bound microRNAs (miRNAs) were exceptionally strong associated with aging. Affected miRNAs regulate the inflammatory response and transcriptional processes, and adipose tissues show considerable effects in associated gene regulatory modules. Finally, nanoparticle tracking and electron microscopy suggest a shift from overall many small to fewer but larger vesicles in aged plasma, potentially contributing to systemic aging trajectories and affecting the molecular aging of organs. |
DOI of the first publication: | 10.1101/2021.05.07.443093 |
URL of the first publication: | https://doi.org/10.1101/2021.05.07.443093 |
Link to this record: | urn:nbn:de:bsz:291--ds-441074 hdl:20.500.11880/39452 http://dx.doi.org/10.22028/D291-44107 |
Date of registration: | 23-Jan-2025 |
Description of the related object: | Supplementary material |
Related object: | https://www.biorxiv.org/content/biorxiv/early/2021/05/08/2021.05.07.443093/DC1/embed/media-1.pdf?download=true https://www.biorxiv.org/content/biorxiv/early/2021/05/08/2021.05.07.443093/DC1/embed/media-1.pdf?download=true https://www.biorxiv.org/content/biorxiv/early/2021/05/08/2021.05.07.443093/DC3/embed/media-3.xlsx?download=true |
Notes: | Preprint |
Faculty: | M - Medizinische Fakultät NT - Naturwissenschaftlich- Technische Fakultät |
Department: | M - Chirurgie M - Humangenetik M - Medizinische Biometrie, Epidemiologie und medizinische Informatik NT - Biowissenschaften NT - Pharmazie |
Professorship: | M - Univ.-Prof. Dr. Andreas Keller M - Prof. Dr. Eckart Meese M - Prof. Dr. Michael D. Menger NT - Jun.-Prof. Dr. Gregor Fuhrmann NT - Prof. Dr. Jörn Walter |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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