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Titel: Adipose Tissue-Derived Microvascular Fragments From Male and Female Fat Donors Exhibit a Comparable Vascularization Capacity
VerfasserIn: Später, Thomas
Marschall, Julia E.
Brücker, Lea K.
Nickels, Ruth M.
Metzger, Wolfgang
Mai, Ann-Sophie
Menger, Michael D.
Laschke, Matthias W.
Sprache: Englisch
Titel: Frontiers in Bioengineering and Biotechnology
Bandnummer: 9
Verlag/Plattform: Frontiers
Erscheinungsjahr: 2021
Freie Schlagwörter: tissue engineering
microvascular fragments
gender
vascularization
angiogenesis
dermal substitute
dorsal skinfold chamber
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Adipose tissue-derived microvascular fragments (MVF) represent effective vascularization units for tissue engineering. Most experimental studies exclusively use epididymal fat tissue of male donor mice as a source for MVF isolation. However, in future clinical practice, MVFbased approaches may be applied in both male and female patients. Therefore, we herein compared the vascularization capacity of MVF isolated from the epididymal and periovarian fat tissue of male and female donor mice. Freshly isolated MVF from male and female donors did not differ in their number, length distribution, viability and cellular composition. After their assembly into spheroids, they also exhibited a comparable in vitro sprouting activity. Moreover, they could be seeded onto collagenglycosaminoglycan matrices, which were implanted into full-thickness skin defects within mouse dorsal skinfold chambers. Repetitive intravital fluorescence microscopy as well as histological and immunohistochemical analyses revealed a comparable vascularization and incorporation of implants seeded with MVF of male and female origin. Taken together, these findings demonstrate that the vascularization capacity of MVF is not gender-specific.
DOI der Erstveröffentlichung: 10.3389/fbioe.2021.777687
URL der Erstveröffentlichung: https://doi.org/10.3389/fbioe.2021.777687
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-440909
hdl:20.500.11880/39442
http://dx.doi.org/10.22028/D291-44090
ISSN: 2296-4185
Datum des Eintrags: 22-Jan-2025
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Chirurgie
Professur: M - Prof. Dr. Michael D. Menger
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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