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Titel: PenTag, a Versatile Platform for Synthesizing Protein‐Polymer Biohybrid Materials
VerfasserIn: Mohsenin, Hasti
Pacheco, Jennifer
Kemmer, Svenja
Wagner, Hanna J.
Höfflin, Nico
Bergmann, Toquinha
Baumann, Tim
Jerez‐Longres, Carolina
Ripp, Alexander
Jork, Nikolaus
Jessen, Henning J.
Fussenegger, Martin
Köhn, Maja
Timmer, Jens
Weber, Wilfried
Sprache: Englisch
Titel: Advanced functional materials
Bandnummer: 34
Heft: 35
Verlag/Plattform: Wiley
Erscheinungsjahr: 2024
Freie Schlagwörter: covalent crosslinking
covalent protein-ligand conjugation
informationprocessing materials
stimuli-responsive hydrogels
DDC-Sachgruppe: 570 Biowissenschaften, Biologie
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: The site-specific and covalent conjugation of proteins on solid supports and in hydrogels is the basis for the synthesis of biohybrid materials offering broad applications. Current methods for conjugating proteins to desired targets are often challenging due to unspecific binding, unstable (noncovalent) coupling, or expensive and difficult-to-synthesize ligand molecules. Here, is presented PenTag, an approach for the bioorthogonal, highly specific, and covalent conjugation of a protein to its ligand for various applications in materials sciences. Penicillin-binding protein 3 (PBP3) is engineered and shows that this protein can be used for the stable and spontaneous conjugation of proteins to dyes, polymers, or solid supports. PenTag as a crosslinking tool is applied for synthesizing stimuli-responsive hydrogels or for the development of a biohybrid material system performing computational operations emulating a 4:2 encoder. Based on this broad applicability and the use of a small, cheap, and easy-to-functionalize ligand and a stable, soluble recombinant protein, is seen PenTag as a versatile approach toward biohybrid material synthesis.
DOI der Erstveröffentlichung: 10.1002/adfm.202308269
URL der Erstveröffentlichung: https://advanced.onlinelibrary.wiley.com/doi/full/10.1002/adfm.202308269
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-440715
hdl:20.500.11880/39432
http://dx.doi.org/10.22028/D291-44071
ISSN: 1616-3028
1616-301X
Datum des Eintrags: 22-Jan-2025
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Prof. Dr. Wilfried Weber
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes



Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons