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Titel: Boosting Tissue Vascularization: Nanofat as a Potential Source of Functional Microvessel Segments
VerfasserIn: Weinzierl, Andrea
Harder, Yves
Schmauss, Daniel
Menger, Michael D.
Laschke, Matthias W.
Sprache: Englisch
Titel: Frontiers in Bioengineering and Biotechnology
Bandnummer: 10
Verlag/Plattform: Frontiers
Erscheinungsjahr: 2022
Freie Schlagwörter: fat graft
nanofat
vascularization
dorsal skinfold chamber
intravital fluorescence microscopy
tissue engineering
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Nanofat is increasingly applied in plastic surgery for the improvement of scar quality and skin rejuvenation. However, little is known about the underlying regenerative mechanisms. Therefore, we herein investigated nanofat grafts in a murine dorsal skinfold chamber model. Nanofat generated from subcutaneous, inguinal adipose tissue of green fluorescent protein (GFP)+ C57BL/6 male and female donor mice was injected intracutaneously into dorsal skinfold chambers of gender-matched GFP− wild-type mice. The vascularization and tissue composition of the grafted nanofat were analyzed by means of intravital fluorescence microscopy, histology and immunohistochemistry over an observation period of 14 days. The freshly generated nanofat consisted of small fragments of perilipin+ adipocytes surrounded by Sirius red+ collagen fibers and still contained intact CD31+ /GFP+ vessel segments. After transplantation into the dorsal skinfold chamber, these vessel segments survived and developed interconnections to the surrounding CD31+ /GFP− host microvasculature. Accordingly, the grafted nanofat rapidly vascularized and formed new microvascular networks with a high functional microvessel density on day 14 without marked differences between male and female mice. Even though further research is needed to confirm these findings, the present study suggests that nanofat boosts tissue vascularization. Thus, nanofat may represent a versatile resource for many applications in tissue engineering and regenerative medicine.
DOI der Erstveröffentlichung: 10.3389/fbioe.2022.820835
URL der Erstveröffentlichung: https://doi.org/10.3389/fbioe.2022.820835
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-440785
hdl:20.500.11880/39431
http://dx.doi.org/10.22028/D291-44078
ISSN: 2296-4185
Datum des Eintrags: 22-Jan-2025
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Chirurgie
Professur: M - Prof. Dr. Michael D. Menger
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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