Bitte benutzen Sie diese Referenz, um auf diese Ressource zu verweisen:
Volltext verfügbar? / Dokumentlieferung
doi:10.22028/D291-44063
Titel: | Establishment of a Reliable Model to Study the Failure of Fracture Healing in Aged Mice |
VerfasserIn: | Menger, Maximilian M. Laschke, Matthias W. Scheuer, Claudia Bauer, David Bleimehl, Michelle Später, Thomas Rollmann, Mika F. Braun, Benedikt J. Herath, Steven C. Raza, Ahsan Menger, Michael D. Histing, Tina |
Sprache: | Englisch |
Titel: | Journals of Gerontology: Biological Sciences |
Bandnummer: | 77 (2022) |
Heft: | 5 |
Seiten: | 909-917 |
Verlag/Plattform: | Oxford University Press |
Erscheinungsjahr: | 2021 |
Freie Schlagwörter: | Aging Fracture healing Inflammation Murine Vascularization |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | The failure of fracture healing represents a substantial clinical problem. Moreover, aged patients demonstrate an elevated risk for failed bone healing. However, murine models to study the failure of fracture healing are established only in young adult animals. Therefore, the aim of this study was to develop a reliable model to study failed fracture healing in aged mice. After creation of a 1.8-mm segmental defect and periosteal resection, femora of aged mice (18–20 months) and young adult control mice (3–4 months) were stabilized by pin-clip fixation. Segmental defects were analyzed by means of biomechanics, x-ray, and micro-computed tomography, as well as histomorphometric, immunohistochemical, and Western blot analysis. After 10 weeks, all animals showed a complete lack of osseous bridging, resulting in fracture healing failure. Segmental defects in aged mice revealed a reduced bone formation and vascularization when compared to young adult mice. This was associated with a decreased expression of bone formation markers. In addition, we detected a reduced number of tartrate-resistant acid phosphatase-positive osteoclasts and an elevated osteoprotegerin/receptor activator of NF-ĸB ligand ratio in aged animals, indicating a reduced osteoclast activity. Moreover, aged animals showed also an enhanced inflammatory response, characterized by an increased infiltration of macrophages within the callus tissue. Taken together, we herein report for the first time a reliable model to study fracture healing failure in aged mice. In the future, the use of this model enables us to study novel therapeutic strategies and molecular mechanics of failed fracture healing during aging. |
DOI der Erstveröffentlichung: | 10.1093/gerona/glab304 |
URL der Erstveröffentlichung: | https://doi.org/10.1093/gerona/glab304 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-440630 hdl:20.500.11880/39420 http://dx.doi.org/10.22028/D291-44063 |
ISSN: | 1758-535X 1079-5006 |
Datum des Eintrags: | 21-Jan-2025 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Chirurgie M - Experimentelle und Klinische Pharmakologie und Toxikologie |
Professur: | M - Prof. Dr. Michael D. Menger M - Prof. Dr. Markus Meyer M - Prof. Dr. Tim Pohlemann |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Es gibt keine Dateien zu dieser Ressource.
Alle Ressourcen in diesem Repository sind urheberrechtlich geschützt.