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Titel: Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis
VerfasserIn: Später, Thomas
Assunção, Marisa
Lit, Kwok Keung
Gong, Guidong
Wang, Xiaoling
Chen, Yi-Yun
Rao, Ying
Li, Yucong
Yiu, Chi Him Kendrick
Laschke, Matthias W.
Menger, Michael D.
Wang, Dan
Tuan, Rocky S
Khoo, Kay-Hooi
Raghunath, Michael
Guo, Junling
Blocki, Anna
Sprache: Englisch
Titel: Bioactive Materials
Bandnummer: 17
Seiten: 526-541
Verlag/Plattform: Elsevier
Erscheinungsjahr: 2022
Freie Schlagwörter: Dextran sulfate
Extracellular matrix
Mesenchymal stem cells
Therapeutic angiogenesis
Wound healing
Poly-electrolyte-driven co-assembly
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Tissue (re)vascularization strategies face various challenges, as therapeutic cells do not survive long enough in situ, while the administration of pro-angiogenic factors is hampered by fast clearance and insufficient ability to emulate complex spatiotemporal signaling. Here, we propose to address these limitations by engineering a functional biomaterial capable of capturing and concentrating the pro-angiogenic activities of mesenchymal stem cells (MSCs). In particular, dextran sulfate, a high molecular weight sulfated glucose polymer, supplemented to MSC cultures, interacts with MSC-derived extracellular matrix (ECM) components and facilitates their co-assembly and accumulation in the pericellular space. Upon decellularization, the resulting dextran sulfate-ECM hybrid material can be processed into MIcroparticles of SOlidified Secretome (MIPSOS). The insoluble format of MIPSOS protects protein components from degradation, while facilitating their sustained release. Proteomic analysis demonstrates that MIPSOS are highly enriched in pro-angiogenic factors, resulting in an enhanced pro-angiogenic bioactivity when compared to naïve MSC-derived ECM (cECM). Consequently, intravital microscopy of full-thickness skin wounds treated with MIPSOS demonstrates accelerated revascularization and healing, far superior to the therapeutic potential of cECM. Hence, the microparticle-based solidified stem cell secretome provides a promising platform to address major limitations of current therapeutic angiogenesis approaches.
DOI der Erstveröffentlichung: 10.1016/j.bioactmat.2022.03.015
URL der Erstveröffentlichung: https://doi.org/10.1016/j.bioactmat.2022.03.015
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-440558
hdl:20.500.11880/39410
http://dx.doi.org/10.22028/D291-44055
ISSN: 2452-199X
Datum des Eintrags: 21-Jan-2025
Bezeichnung des in Beziehung stehenden Objekts: Supplementary data
In Beziehung stehendes Objekt: https://ars.els-cdn.com/content/image/1-s2.0-S2452199X2200130X-mmc1.docx
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Chirurgie
Professur: M - Prof. Dr. Michael D. Menger
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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