Bitte benutzen Sie diese Referenz, um auf diese Ressource zu verweisen:
Volltext verfügbar? / Dokumentlieferung
doi:10.22028/D291-43672
Titel: | A screening setup to streamline in vitro engineered living material cultures with the host |
VerfasserIn: | Desai, Krupansh Sankaran, Shrikrishnan del Campo Bécares, Aránzazu Trujillo, Sara |
Sprache: | Englisch |
Verlag/Plattform: | bioRxiv |
Erscheinungsjahr: | 2024 |
Freie Schlagwörter: | Engineered living materials living therapeutics biocompatibility in vitro culture |
DDC-Sachgruppe: | 540 Chemie |
Dokumenttyp: | Sonstiges |
Abstract: | Engineered living materials (ELMs), which usually comprise bacteria, fungi, or animal cells entrapped in polymeric matrices, offer limitless possibilities in fields like drug delivery or biosensing. To determine the conditions that sustain ELM performance while ensuring ELM-host compatibility is essential before testing them in vivo. This is critical to reduce animal experimentation and can be achieved through in vitro investigations. Towards this goal, we designed a 96-well plate-based screening method to streamline ELM growth across culture conditions and determine their compatibility potential in vitro. We showed proliferation of three bacterial species encapsulated in hydrogels over time and screened six different cell culture media. We fabricated ELMs in bilayer and monolayer formats and tracked bacterial leakage. After screening, an appropriate medium was selected that sustained growth of an ELM, and it was used to study cytocompatibility in vitro. ELM cytotoxicity on murine fibroblasts and human monocytes was studied by adding ELM supernatants and measuring cell membrane integrity and live/dead staining, respectively, proving ELM cytocompatibility. Our work illustrates a simple setup to streamline the screening of compatible environmental conditions of ELMs with the host.Competing Interest StatementThe authors have declared no competing interest. |
DOI der Erstveröffentlichung: | 10.1101/2024.08.22.609167 |
URL der Erstveröffentlichung: | https://www.biorxiv.org/content/10.1101/2024.08.22.609167v1 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-436728 hdl:20.500.11880/39142 http://dx.doi.org/10.22028/D291-43672 |
Datum des Eintrags: | 10-Dez-2024 |
Bemerkung/Hinweis: | Preprint |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Chemie |
Professur: | NT - Prof. Dr. Aránzazu del Campo |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Es gibt keine Dateien zu dieser Ressource.
Alle Ressourcen in diesem Repository sind urheberrechtlich geschützt.