Bitte benutzen Sie diese Referenz, um auf diese Ressource zu verweisen: doi:10.22028/D291-43672
Volltext verfügbar? / Dokumentlieferung
Titel: A screening setup to streamline in vitro engineered living material cultures with the host
VerfasserIn: Desai, Krupansh
Sankaran, Shrikrishnan
del Campo Bécares, Aránzazu
Trujillo, Sara
Sprache: Englisch
Verlag/Plattform: bioRxiv
Erscheinungsjahr: 2024
Freie Schlagwörter: Engineered living materials
living therapeutics
biocompatibility
in vitro culture
DDC-Sachgruppe: 540 Chemie
Dokumenttyp: Sonstiges
Abstract: Engineered living materials (ELMs), which usually comprise bacteria, fungi, or animal cells entrapped in polymeric matrices, offer limitless possibilities in fields like drug delivery or biosensing. To determine the conditions that sustain ELM performance while ensuring ELM-host compatibility is essential before testing them in vivo. This is critical to reduce animal experimentation and can be achieved through in vitro investigations. Towards this goal, we designed a 96-well plate-based screening method to streamline ELM growth across culture conditions and determine their compatibility potential in vitro. We showed proliferation of three bacterial species encapsulated in hydrogels over time and screened six different cell culture media. We fabricated ELMs in bilayer and monolayer formats and tracked bacterial leakage. After screening, an appropriate medium was selected that sustained growth of an ELM, and it was used to study cytocompatibility in vitro. ELM cytotoxicity on murine fibroblasts and human monocytes was studied by adding ELM supernatants and measuring cell membrane integrity and live/dead staining, respectively, proving ELM cytocompatibility. Our work illustrates a simple setup to streamline the screening of compatible environmental conditions of ELMs with the host.Competing Interest StatementThe authors have declared no competing interest.
DOI der Erstveröffentlichung: 10.1101/2024.08.22.609167
URL der Erstveröffentlichung: https://www.biorxiv.org/content/10.1101/2024.08.22.609167v1
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-436728
hdl:20.500.11880/39142
http://dx.doi.org/10.22028/D291-43672
Datum des Eintrags: 10-Dez-2024
Bemerkung/Hinweis: Preprint
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Chemie
Professur: NT - Prof. Dr. Aránzazu del Campo
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Dateien zu diesem Datensatz:
Es gibt keine Dateien zu dieser Ressource.


Alle Ressourcen in diesem Repository sind urheberrechtlich geschützt.