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Titel: Signal-amplifying Biohybrid Material Circuits for CRISPR/Cas-based single-stranded RNA Detection
VerfasserIn: Mohsenin, Hasti
Schmachtenberg, Rosanne
Kemmer, Svenja
Wagner, Hanna J.
Johnston, Midori
Madlener, Sibylle
Dincer, Can
Timmer, Jens
Weber, Wilfried
Sprache: Englisch
Verlag/Plattform: medRxiv
Erscheinungsjahr: 2024
Freie Schlagwörter: biosensing
CRISPR/Cas-powered diagnostics
information processing materials
mathematical modelling
miRNA
ssRNA
synthetic biology
DDC-Sachgruppe: 570 Biowissenschaften, Biologie
610 Medizin, Gesundheit
Dokumenttyp: Sonstiges
Abstract: The functional integration of biological switches with synthetic buildingblocks enables the design of modular, stimulus-responsive biohybridmaterials. By connecting the individual modules via diffusible signals,information-processing circuits can be designed. Such systems are, however,mostly limited to respond to either small molecules, proteins, or optical inputthus limiting the sensing and application scope of the material circuits. Here,a highly modular biohybrid material is design based on CRISPR/Cas13a totranslate arbitrary single-stranded RNAs into a biomolecular material response.This system exemplified by the development of a cascade of communicatingmaterials that can detect the tumor biomarker microRNA miR19b in patientsamples or sequences specific for SARS-CoV. Specificity of the system is furtherdemonstrated by discriminating between input miRNA sequences with single-nucleotide differences. To quantitatively understand information processing inthe materials cascade, a mathematical model is developed. The model is usedto guide systems design for enhancing signal amplification functionality of theoverall materials system. The newly designed modular materials can be usedto interface desired RNA input with stimulus-responsive and information-processing materials for building point-of-care suitable sensors as well as multi-input diagnostic systems with integrated data processing and interpretation.
DOI der Erstveröffentlichung: 10.1101/2024.06.12.24308852
URL der Erstveröffentlichung: https://www.medrxiv.org/content/10.1101/2024.06.12.24308852v1
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-436647
hdl:20.500.11880/39132
http://dx.doi.org/10.22028/D291-43664
Datum des Eintrags: 6-Dez-2024
Bemerkung/Hinweis: Preprint
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Prof. Dr. Wilfried Weber
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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