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Titel: Cell Free Expression in Proteinosomes Prepared from Native Protein-PNIPAAm Conjugates
VerfasserIn: Gao, Mengfei
Wang, Dishi
Wilsch-Bräuninger, Michaela
Leng, Weihua
Schulte, Jonathan
Morgner, Nina
Appelhans, Dietmar
Tang, T-Y Dora
Sprache: Englisch
In:
Titel: Macromolecular bioscience
Bandnummer: 24
Heft: 3
Verlag/Plattform: Wiley
Erscheinungsjahr: 2024
DDC-Sachgruppe: 570 Biowissenschaften, Biologie
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Towards the goal of building synthetic cells from the bottom-up, the establishment of micrometer-sized compartments that contain and support cell free transcription and translation that couple cellular structure to function is of critical importance. Proteinosomes, formed from crosslinked cationized protein-polymer conjugates offer a promising solution to membrane-bound compartmentalization with an open, semi-permeable membrane. Critically, to date, there has been no demonstration of cell free transcription and translation within water-in-water proteinosomes. Herein, a novel approach to generate proteinosomes that can support cell free transcription and translation is presented. This approach generates proteinosomes directly from native protein-polymer (BSA-PNIPAAm) conjugates. These native proteinosomes offer an excellent alternative as a synthetic cell chassis to other membrane bound compartments. Significantly, the native proteinosomes are stable under high salt conditions that enables the ability to support cell free transcription and translation and offer enhanced protein expression compared to proteinosomes prepared from traditional methodologies. Furthermore, the integration of native proteinosomes into higher order synthetic cellular architectures with membrane free compartments such as liposomes is demonstrated. The integration of bioinspired architectural elements with the central dogma is an essential building block for realizing minimal synthetic cells and is key for exploiting artificial cells in real-world applications.
DOI der Erstveröffentlichung: 10.1002/mabi.202300464
URL der Erstveröffentlichung: https://onlinelibrary.wiley.com/doi/10.1002/mabi.202300464
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-436609
hdl:20.500.11880/39125
http://dx.doi.org/10.22028/D291-43660
ISSN: 1616-5195
1616-5187
Datum des Eintrags: 5-Dez-2024
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Keiner Professur zugeordnet
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes



Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons