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doi:10.22028/D291-43650
Titel: | Oxidative stress in metabolic dysfunction-associated steatotic liver disease (MASLD): How does the animal model resemble human disease? |
VerfasserIn: | Jakubek, Patrycja Kalinowski, Piotr Karkucinska-Wieckowska, Agnieszka Kaikini, Aakruti Simões, Inês C. M. Potes, Yaiza Kruk, Beata Grajkowska, Wieslawa Pinton, Paolo Milkiewicz, Piotr Grąt, Michał Pronicki, Maciej Lebiedzinska-Arciszewska, Magdalena Krawczyk, Marcin Jan Wieckowski, Mariusz R. |
Sprache: | Englisch |
Titel: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology |
Bandnummer: | 38 |
Heft: | 3 |
Verlag/Plattform: | Wiley |
Erscheinungsjahr: | 2024 |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Despite decades of research, the pathogenesis of metabolic dysfunction-associated steatotic liver disease (MASLD) is still not completely understood. Based on the evidence from preclinical models, one of the factors proposed as a main driver of disease development is oxidative stress. This study aimed to search for the resemblance between the profiles of oxidative stress and antioxidant defense in the animal model of MASLD and the group of MASLD patients. C57BL/6J mice were fed with the Western diet for up to 24 weeks and served as the animal model of MASLD. The antioxidant profile of mice hepatic tissue was determined by liquid chromatography-MS3 spectrometry (LC-MS/MS). The human cohort consisted of 20 patients, who underwent bariatric surgery, and 6 controls. Based on histological analysis, 4 bariatric patients did not have liver steatosis and as such were also classified as controls. Total antioxidant activity was measured in sera and liver biopsy samples. The hepatic levels of antioxidant enzymes and oxidative damage were determined by Western Blot. The levels of antioxidant enzymes were significantly altered in the hepatic tissue of mice with MASLD. In contrast, there were no significant changes in the antioxidant profile of hepatic tissue of MASLD patients, except for the decreased level of carbonylated proteins. Decreased protein carbonylation together with significant correlations between the thioredoxin system and parameters describing metabolic health suggest alterations in the thiol-redox signaling. Altogether, these data show that even though the phenotype of mice closely resembles human MASLD, the animal-to-human translation of cellular and molecular processes such as oxidative stress may be more challenging. |
DOI der Erstveröffentlichung: | 10.1096/fj.202302447R |
URL der Erstveröffentlichung: | https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202302447R |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-436501 hdl:20.500.11880/39116 http://dx.doi.org/10.22028/D291-43650 |
ISSN: | 1530-6860 0892-6638 |
Datum des Eintrags: | 4-Dez-2024 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Innere Medizin |
Professur: | M - Keiner Professur zugeordnet |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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The FASEB Journal - 2024 - Jakubek - Oxidative stress in metabolic dysfunction‐associated steatotic liver disease MASLD .pdf | 4,03 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons