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doi:10.22028/D291-43544
Titel: | Uric acid detection by hydrogen peroxide independent biosensors: Novel insights and applications |
VerfasserIn: | Koch, Marcus Silina, Yuliya |
Sprache: | Englisch |
Titel: | Microchemical journal |
Bandnummer: | 207 |
Verlag/Plattform: | Elsevier |
Erscheinungsjahr: | 2024 |
Freie Schlagwörter: | Uric acid Uricase Electrodeposition Biochemical route Electrooxidation Fermentation samples |
DDC-Sachgruppe: | 500 Naturwissenschaften |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Uric acid (UA) is one of the most electroactive low molecular weight compounds that can be electrochemically oxidized on the surfaces of numerous noble and non-noble electrocatalysts under applied polarization. Consequently, enzymatic determination of UA in model and real samples is complicated by possible interference between electrochemical and biochemical routes. Herein, a novel strategy for amperometric enzymatic hydrogen peroxide independent UA sensing at low concentrations (e.g., below 50 µM) is proposed. The UA-sensitive strategy relies on the use of screen printed electrodes modified by an electrodeposited hybrid functional sensing film comprising a non-noble electrocatalyst, a bioorganic layer containing enzyme uricase (UOx), and data acquisition enabling the biochemical transformation of UA to be distinguished from the electrochemical oxidation route. Performed selectivity test utilizing adenine, xanthine, urea, ascorbic acid, ethanol and glycerol did not reveal interferences during detection of UA. This proposed approach was tested for UA detection in model and fermentation samples. The quantitative results obtained in fermentation samples were validated through optical oxygen mini sensor studies and fluorescence-based bioassays. |
DOI der Erstveröffentlichung: | 10.1016/j.microc.2024.112091 |
URL der Erstveröffentlichung: | https://www.sciencedirect.com/science/article/pii/S0026265X24022033 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-435448 hdl:20.500.11880/39079 http://dx.doi.org/10.22028/D291-43544 |
ISSN: | 0026-265X |
Datum des Eintrags: | 2-Dez-2024 |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Biowissenschaften |
Professur: | NT - Prof. Dr. Bruce Morgan |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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1-s2.0-S0026265X24022033-main.pdf | 2,55 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons