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Titel: Impact of Drug Administration Routes on the In Vivo Efficacy of the Natural Product Sorangicin a Using a Staphylococcus aureus Infection Model in Zebrafish Embryos
VerfasserIn: Fries, Franziska
Kany, Andreas M.
Rasheed, Sari Shawki
Hirsch, Anna
Müller, Rolf
Herrmann, Jennifer
Sprache: Englisch
Titel: International journal of molecular sciences
Bandnummer: 24
Heft: 16
Verlag/Plattform: MDPI
Erscheinungsjahr: 2023
Freie Schlagwörter: zebrafish
Staphylococcus aureus
infection
drug discovery
in vivo efficacy
sorangicin
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Staphylococcus aureus causes a wide range of infections, and it is one of the leading pathogens responsible for deaths associated with antimicrobial resistance, the rapid spread of which among S. aureus urges the discovery of new antibiotics. The evaluation of in vivo efficacy of novel drug candidates is usually performed using animal models. Recently, zebrafish (Danio rerio) embryos have become increasingly attractive in early drug discovery. Herein, we established a zebrafish embryo model of S. aureus infection for evaluation of in vivo efficacy of novel potential antimicrobials. A local infection was induced by microinjecting mCherry-expressing S. aureus Newman followed by treatment with reference antibiotics via microinjection into different injection sites as well as via waterborne exposure to study the impact of the administration route on efficacy. We successfully used the developed model to evaluate the in vivo activity of the natural product sorangicin A, for which common mouse models were not successful due to fast degradation in plasma. In conclusion, we present a novel screening platform for assessing in vivo activity at the antibiotic discovery stage. Furthermore, this work provides consideration for the choice of an appropriate administration route based on the physicochemical properties of tested drugs.
DOI der Erstveröffentlichung: 10.3390/ijms241612791
URL der Erstveröffentlichung: https://www.mdpi.com/1422-0067/24/16/12791
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-435266
hdl:20.500.11880/39011
http://dx.doi.org/10.22028/D291-43526
ISSN: 1422-0067
Datum des Eintrags: 21-Nov-2024
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Pharmazie
Professur: NT - Prof. Dr. Anna Hirsch
NT - Prof. Dr. Rolf Müller
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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