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doi:10.22028/D291-43526
Titel: | Impact of Drug Administration Routes on the In Vivo Efficacy of the Natural Product Sorangicin a Using a Staphylococcus aureus Infection Model in Zebrafish Embryos |
VerfasserIn: | Fries, Franziska Kany, Andreas M. Rasheed, Sari Shawki Hirsch, Anna Müller, Rolf Herrmann, Jennifer |
Sprache: | Englisch |
Titel: | International journal of molecular sciences |
Bandnummer: | 24 |
Heft: | 16 |
Verlag/Plattform: | MDPI |
Erscheinungsjahr: | 2023 |
Freie Schlagwörter: | zebrafish Staphylococcus aureus infection drug discovery in vivo efficacy sorangicin |
DDC-Sachgruppe: | 500 Naturwissenschaften |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Staphylococcus aureus causes a wide range of infections, and it is one of the leading pathogens responsible for deaths associated with antimicrobial resistance, the rapid spread of which among S. aureus urges the discovery of new antibiotics. The evaluation of in vivo efficacy of novel drug candidates is usually performed using animal models. Recently, zebrafish (Danio rerio) embryos have become increasingly attractive in early drug discovery. Herein, we established a zebrafish embryo model of S. aureus infection for evaluation of in vivo efficacy of novel potential antimicrobials. A local infection was induced by microinjecting mCherry-expressing S. aureus Newman followed by treatment with reference antibiotics via microinjection into different injection sites as well as via waterborne exposure to study the impact of the administration route on efficacy. We successfully used the developed model to evaluate the in vivo activity of the natural product sorangicin A, for which common mouse models were not successful due to fast degradation in plasma. In conclusion, we present a novel screening platform for assessing in vivo activity at the antibiotic discovery stage. Furthermore, this work provides consideration for the choice of an appropriate administration route based on the physicochemical properties of tested drugs. |
DOI der Erstveröffentlichung: | 10.3390/ijms241612791 |
URL der Erstveröffentlichung: | https://www.mdpi.com/1422-0067/24/16/12791 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-435266 hdl:20.500.11880/39011 http://dx.doi.org/10.22028/D291-43526 |
ISSN: | 1422-0067 |
Datum des Eintrags: | 21-Nov-2024 |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Pharmazie |
Professur: | NT - Prof. Dr. Anna Hirsch NT - Prof. Dr. Rolf Müller |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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ijms-24-12791-v5.pdf | 2,31 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons