Please use this identifier to cite or link to this item: doi:10.22028/D291-42644
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Title: Two Piwis with Ago-like functions silence somatic genes at the chromatin level
Author(s): Drews, Franziska
Karunanithi, Sivarajan
Götz, Ulrike
Marker, Simone
deWijn, Raphael
Pirritano, Marcello
Rodrigues-Viana, Angela M.
Jung, Martin
Gasparoni, Gilles
Schulz, Marcel H.
Simon, Martin
Language: English
Title: RNA Biology
Volume: 18
Issue: sup2
Pages: 757-769
Publisher/Platform: Taylor & Francis
Year of Publication: 2021
Free key words: Argonaute
piwi
sRNA loading
RNA interference
siRNA
dicer
transgeneinduced silencing
secondary siRNAs
DDC notations: 500 Science
610 Medicine and health
Publikation type: Journal Article
Abstract: Most sRNA biogenesis mechanisms involve either RNAse III cleavage or ping-pong amplification by different Piwi proteins harbouring slicer activity. Here, we follow the question why the mechanism of transgene-induced silencing in the ciliate Paramecium needs both Dicer activity and two Ptiwi proteins. This pathway involves primary siRNAs produced from non-translatable transgenes and secondary siRNAs from targeted endogenous loci. Our data does not indicate any signatures from ping-pong amplification but Dicer cleavage of long dsRNA. Ptiwi13 and 14 prefer different sub-cellular localizations and different preferences for primary and secondary siRNAs but do not load them mutually exclusive. Both Piwis enrich for antisense RNAs and show a general preference for uridine-rich sRNAs along the entire sRNA length. In addition, Ptiwi14-loaded siRNAs show a 5´-U signature. Our data indicates both Ptiwis and 2 ´-O-methylation contributing to strand selection of Dicer cleaved siRNAs. This unexpected function of the two distinct vegetative Piwis extends the increasing knowledge of the diversity of Piwi functions in diverse silencing pathways. We describe an unusual mode of action of Piwi proteins extending not only the great variety of Piwi-associated RNAi pathways but moreover raising the question whether this could have been the primordial one.
DOI of the first publication: 10.1080/15476286.2021.1991114
URL of the first publication: https://doi.org/10.1080/15476286.2021.1991114
Link to this record: urn:nbn:de:bsz:291--ds-426448
hdl:20.500.11880/38247
http://dx.doi.org/10.22028/D291-42644
ISSN: 1555-8584
1547-6286
Date of registration: 12-Aug-2024
Description of the related object: Supplemental material
Related object: https://ndownloader.figstatic.com/files/31120069
Faculty: M - Medizinische Fakultät
NT - Naturwissenschaftlich- Technische Fakultät
Department: M - Medizinische Biochemie und Molekularbiologie
NT - Biowissenschaften
Professorship: M - Prof. Dr. Martin Van der Laan
NT - Prof. Dr. Jörn Walter
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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