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doi:10.22028/D291-42644
Title: | Two Piwis with Ago-like functions silence somatic genes at the chromatin level |
Author(s): | Drews, Franziska Karunanithi, Sivarajan Götz, Ulrike Marker, Simone deWijn, Raphael Pirritano, Marcello Rodrigues-Viana, Angela M. Jung, Martin Gasparoni, Gilles Schulz, Marcel H. Simon, Martin |
Language: | English |
Title: | RNA Biology |
Volume: | 18 |
Issue: | sup2 |
Pages: | 757-769 |
Publisher/Platform: | Taylor & Francis |
Year of Publication: | 2021 |
Free key words: | Argonaute piwi sRNA loading RNA interference siRNA dicer transgeneinduced silencing secondary siRNAs |
DDC notations: | 500 Science 610 Medicine and health |
Publikation type: | Journal Article |
Abstract: | Most sRNA biogenesis mechanisms involve either RNAse III cleavage or ping-pong amplification by different Piwi proteins harbouring slicer activity. Here, we follow the question why the mechanism of transgene-induced silencing in the ciliate Paramecium needs both Dicer activity and two Ptiwi proteins. This pathway involves primary siRNAs produced from non-translatable transgenes and secondary siRNAs from targeted endogenous loci. Our data does not indicate any signatures from ping-pong amplification but Dicer cleavage of long dsRNA. Ptiwi13 and 14 prefer different sub-cellular localizations and different preferences for primary and secondary siRNAs but do not load them mutually exclusive. Both Piwis enrich for antisense RNAs and show a general preference for uridine-rich sRNAs along the entire sRNA length. In addition, Ptiwi14-loaded siRNAs show a 5´-U signature. Our data indicates both Ptiwis and 2 ´-O-methylation contributing to strand selection of Dicer cleaved siRNAs. This unexpected function of the two distinct vegetative Piwis extends the increasing knowledge of the diversity of Piwi functions in diverse silencing pathways. We describe an unusual mode of action of Piwi proteins extending not only the great variety of Piwi-associated RNAi pathways but moreover raising the question whether this could have been the primordial one. |
DOI of the first publication: | 10.1080/15476286.2021.1991114 |
URL of the first publication: | https://doi.org/10.1080/15476286.2021.1991114 |
Link to this record: | urn:nbn:de:bsz:291--ds-426448 hdl:20.500.11880/38247 http://dx.doi.org/10.22028/D291-42644 |
ISSN: | 1555-8584 1547-6286 |
Date of registration: | 12-Aug-2024 |
Description of the related object: | Supplemental material |
Related object: | https://ndownloader.figstatic.com/files/31120069 |
Faculty: | M - Medizinische Fakultät NT - Naturwissenschaftlich- Technische Fakultät |
Department: | M - Medizinische Biochemie und Molekularbiologie NT - Biowissenschaften |
Professorship: | M - Prof. Dr. Martin Van der Laan NT - Prof. Dr. Jörn Walter |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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