Please use this identifier to cite or link to this item: doi:10.22028/D291-42637
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Title: Peptidic biofunctionalization of laser patterned dental zirconia: A biochemical-topographical approach
Author(s): Minguela, J.
Müller, D. W.
Mücklich, F.
Llanes, L.
Ginebra, M. P.
Roa, J. J.
Mas-Moruno, C.
Language: English
Title: Materials Science & Engineering
Volume: 125
Publisher/Platform: Elsevier
Year of Publication: 2021
Free key words: Zirconia
Laser patterning
Surface functionalization
Peptides
Osteointegration
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: A dual approach employing peptidic biofunctionalization and laser micro-patterns on dental zirconia was explored, with the aim of providing a flexible tool to improve tissue integration of restorations. Direct laser interference patterning with a femtosecond Ti:Sapphire laser was employed, and two periodic grooved patterns were produced with a periodicity of 3 and 10 μm. A platform containing the cell-adhesive RGD and the osteogenic DWIVA peptides was used to functionalize the grooved surfaces. Topography and surface damage were characterized by confocal laser scanning (CLSM), scanning electron and scanning transmission electron microscopy techniques. The surface patterns exhibited a high homogeneity and subsurface damage was found in the form of nano-cracks and nano-pores, at the bottom of the valleys. Accelerated tests in water steam were carried out to assess hydrothermal degradation resistance, which slightly decreased after the laser treatment. Interestingly, the detrimental effects of the laser modification were reverted by a post-laser thermal treatment. The attachment of the molecule was verified trough fluorescence CLSM and X-ray photoelectron spectroscopy. Finally, the biological properties of the surfaces were studied in human mesenchymal stem cells. Cell adhesion, morphology, migration and differentiation were investigated. Cells on grooved surfaces displayed an elongated morphology and aligned along the patterns. On these surfaces, migration was greatly enhanced along the grooves, but also highly restricted in the perpendicular direction as compared to flat specimens. After biofunctionalization, cell number and cell area increased and well-developed cell cytoskeletons were observed. However, no effects on cell migration were found for the peptidic platform. Although some osteogenic potential was found in specimens grooved with a periodicity of 10 μm, the largest effects were observed from the biomolecule, which favored upregulation of several genes related to osteoblastic differentiation in all the surfaces.
DOI of the first publication: 10.1016/j.msec.2021.112096
URL of the first publication: https://doi.org/10.1016/j.msec.2021.112096
Link to this record: urn:nbn:de:bsz:291--ds-426370
hdl:20.500.11880/38240
http://dx.doi.org/10.22028/D291-42637
ISSN: 0928-4931
Date of registration: 12-Aug-2024
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Materialwissenschaft und Werkstofftechnik
Professorship: NT - Prof. Dr. Frank Mücklich
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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