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Titel: Application-Oriented Bulk Cryopreservation of Human iPSCs in Cryo Bags Followed by Direct Inoculation in Scalable Suspension Bioreactors for Expansion and Neural Differentiation
VerfasserIn: Meiser, Ina
Alstrup, Monica
Khalesi, Elham
Stephan, Bianca
Speicher, Anna M.
Majer, Julia
Kwok, Chee Keong
Neubauer, Julia C.
Hansson, Mattias
Zimmermann, Heiko
Sprache: Englisch
Titel: Cells : open access journal
Bandnummer: 12
Heft: 14
Seiten: 1-19
Verlag/Plattform: MDPI
Erscheinungsjahr: 2023
Freie Schlagwörter: hiPSC
cryopreservation
suspension bioreactors
cell therapy
bulk
expansion
differentiation
neurons
cryo bag
DDC-Sachgruppe: 570 Biowissenschaften, Biologie
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Stem cell-based therapies are promising tools for regenerative medicine and require bulk numbers of high-quality cells. Currently, cells are produced on demand and have a limited shelf-life as conventional cryopreservation is primarily designed for stock keeping. We present a study on bulk cryopreservation of the human iPSC lines UKKi011-A and BIONi010-C-41. By increasing cell concentration and volume, compared to conventional cryopreservation routines in cryo vials, one billion cells were frozen in 50 mL cryo bags. Upon thawing, the cells were immediately seeded in scalable suspension-based bioreactors for expansion to assess the stemness maintenance and for neural differentiation to assess their differentiation potential on the gene and protein levels. Both the conventional and bulk cryo approach show comparative results regarding viability and aggregation upon thawing and bioreactor inoculation. Reduced performance compared to the non-frozen control was compensated within 3 days regarding biomass yield. Stemness was maintained upon thawing in expansion. In neural differentiation, a delay of the neural marker expression on day 4 was compensated at day 9. We conclude that cryopreservation in cryo bags, using high cell concentrations and volumes, does not alter the cells' fate and is a suitable technology to avoid pre-cultivation and enable time- and cost-efficient therapeutic approaches with bulk cell numbers.
DOI der Erstveröffentlichung: 10.3390/cells12141914
URL der Erstveröffentlichung: https://www.mdpi.com/2073-4409/12/14/1914
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-425606
hdl:20.500.11880/38183
http://dx.doi.org/10.22028/D291-42560
ISSN: 2073-4409
Datum des Eintrags: 5-Aug-2024
Drittmittel / Förderung: EU()
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Prof. Dr. Heiko Zimmermann
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons