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Titel: 3D bioprinting ofE. coliMG1655 biofilms on human lung epithelial cells for building complexin vitroinfection models
VerfasserIn: Aliyazdi, Samy
Frisch, Sarah
Hidalgo, Alberto
Frank, Nicolas Alexander
Krug, Daniel
Müller, Rolf
Schaefer, Ulrich F.
Vogt, Thomas
Loretz, Brigitta
Lehr, Claus-Michael
Sprache: Englisch
Titel: Biofabrication
Bandnummer: 15
Heft: 3
Seiten: 1-14
Verlag/Plattform: IOP Publishing
Erscheinungsjahr: 2023
Freie Schlagwörter: 3D printing
hydrogel
antimicrobial resistance
alternatives to animal experiments
chronic lung infections
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Biofilm-associated infections are causing over half a million deaths each year, raising the requirement for innovative therapeutic approaches. For developing novel therapeutics against bacterial biofilm infections, complexin vitromodels that allow to study drug effects on both pathogens and host cells as well as their interaction under controlled, physiologically relevant conditions appear as highly desirable. Nonetheless, building such models is quite challenging because (1) rapid bacterial growth and release of virulence factors may lead to premature host cell death and (2) maintaining the biofilm status under suitable co-culture requires a highly controlled environment. To approach that problem, we chose 3D bioprinting. However, printing living bacterial biofilms in defined shapes on human cell models, requires bioinks with very specific properties. Hence, this work aims to develop a 3D bioprinting biofilm method to build robustin vitroinfection models. Based on rheology, printability and bacterial growth, a bioink containing 3% gelatin and 1% alginate in Luria-Bertani-medium was found optimal forEscherichia coliMG1655 biofilms. Biofilm properties were maintained after printing, as shown visually via microscopy techniques as well as in antibiotic susceptibility assays. Metabolic profile analysis of bioprinted biofilms showed high similarity to native biofilms. After printing on human bronchial epithelial cells (Calu-3), the shape of printed biofilms was maintained even after dissolution of non-crosslinked bioink, while no cytotoxicity was observed over 24 h. Therefore, the approach presented here may provide a platform for building complexin vitroinfection models comprising bacterial biofilms and human host cells.
DOI der Erstveröffentlichung: 10.1088/1758-5090/acd95e
URL der Erstveröffentlichung: https://iopscience.iop.org/article/10.1088/1758-5090/acd95e
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-425508
hdl:20.500.11880/38174
http://dx.doi.org/10.22028/D291-42550
ISSN: 1758-5090
1758-5082
Datum des Eintrags: 5-Aug-2024
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Pharmazie
Professur: NT - Prof. Dr. Claus-Michael Lehr
NT - Prof. Dr. Rolf Müller
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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