Please use this identifier to cite or link to this item: doi:10.22028/D291-42369
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Title: Thickness and Curvature Changes of Human Corneal Grafts in Dextran-Containing Organ Culture Medium Before Keratoplasty
Author(s): Hamon, Loïc
Daas, Loay
Mäurer, Stephanie
Weinstein, Isabel
Quintin, Adrien
Schulz, Katja
Langenbucher, Achim
Seitz, Berthold
Language: English
Title: Cornea
Volume: 40 (2021)
Issue: 6
Pages: 733-740
Publisher/Platform: Lippincott, Williams & Wilkins
Year of Publication: 2020
Free key words: cornea
eye bank
dextran
deswelling
penetrating keratoplasty
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Purpose: To determine the changes of corneal thickness and curvature of human corneal grafts in organ culture medium II, containing dextran T500 6%, before keratoplasty. Methods: We examined the tomography of 24 corneas from our eye bank transferred from medium I into medium II. Images were repeated hourly during 24 hours using an anterior segment optical coherence tomography. The central corneal thickness (CCT) was measured with the manual measurement tool of the anterior segment optical coherence tomography. The radii of curvature (anterior flat and steep and posterior flat and steep) were measured with a MATLAB self-programmed software for “sterile donor tomography.” Results: The mean CCT (6SD) at baseline (T0) was 727 6 156 mm. It reached 581 6 103, 506 6 84, 472 6 79, and 45667 mm after 6, 12, 18, and 24 hours, respectively. After 12 hours, 83% of the final deswelling was achieved. The radii of curvature (6SD) at baseline (T0) were (posterior flat, posterior steep, anterior flat, and anterior steep) 6.6 6 0.5, 6.2 6 0.5, 7.7 6 0.4, and 7.4 6 0.4 mm, respectively. After 24 hours, the radii of curvature reached 6.8 6 0.1, 6.6 6 0.3, 7.6 6 0.1, and 7.4 6 0.2 mm, respectively. Conclusions: The kinetics of the deswelling process in medium II follow a hyperbolic curve. Considering a CCT of 506 mm at T12, we assume that a time interval of 12 hours in medium II might be enough for clinical purposes. This result might help to keep storage in medium II as short as possible to escape potential toxic effects of dextran in medium II. The radius of curvature does not seem to change within 24 hours for all measured surfaces.
DOI of the first publication: 10.1097/ICO.0000000000002543
URL of the first publication: https://journals.lww.com/corneajrnl/fulltext/2021/06000/thickness_and_curvature_changes_of_human_corneal.11.aspx
Link to this record: urn:nbn:de:bsz:291--ds-423699
hdl:20.500.11880/38031
http://dx.doi.org/10.22028/D291-42369
ISSN: 0277-3740
Date of registration: 11-Jul-2024
Faculty: M - Medizinische Fakultät
Department: M - Augenheilkunde
Professorship: M - Univ.-Prof. Dr. Dipl.-Ing. Achim Langenbucher
M - Prof. Dr. Berthold Seitz
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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