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Titel: Deletion of nicotinic acetylcholine receptor alpha9 in mice resulted in altered bone structure
VerfasserIn: Baumann, Lisa
Kauschke, Vivien
Vikman, Anna
Dürselen, Lutz
Krasteva-Christ, Gabriela
Kampschulte, Marian
Heiss, Christian
Yee, Kathleen T.
Vetter, Douglas E.
Lips, Katrin Susanne
Sprache: Englisch
Titel: Bone
Bandnummer: 120 (2019)
Seiten: 285-296
Verlag/Plattform: Elsevier
Erscheinungsjahr: 2018
Freie Schlagwörter: Bending stiffness
Micro-CT
Connexin-43
Collagen 1α1
nAChR α10
Non-neuronal cholinergic system
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Alterations in bone strength and structure were found in knockout (KO) mouse strains with deletion of several acetylcholine receptors. Interestingly, the expression of the nicotinic acetylcholine receptors (nAChR) subunit α10 was down-regulated in osteogenic differentiated mesenchymal stem cells of patients with osteoporosis whereas the expression of subunit α9 was not altered. Since nAChR subunits α9 and α10 are often combined in a functional receptor, we analyzed here the bone of adult female KO mice with single deletion of either nAChR alpha9 (α9KO) or alpha10 (α10KO). Biomechanical testing showed a significant decrease of bending stiffness and maximal breaking force in α9KO compared to their corresponding wild type mice. Furthermore, an increase in trabecular pattern factor (Tb.Pf) and structure model index (SMI) was detected by μCT in α9KO indicating reduced bone mass. On the mRNA level a decrease of Collagen 1α1 and Connexin-43 was measured by real-time RT-PCR in α9KO while no alteration of osteoclast markers was detected in either mouse strain. Using electron microcopy we observed an increase in the number of osteocytes that showed signs of degeneration and cell death in the α9KO compared to their wild type mice, while α10KO showed no differences. In conclusion, we demonstrate alterations in bone strength, structure and bio-marker expression in α9KO mice which imply the induction of osteocyte degeneration. Thus, our data suggest that nAChR containing the α9 subunit might be involved in the homeostasis of osteocytes and therefore in bone mass regulation.
DOI der Erstveröffentlichung: 10.1016/j.bone.2018.11.003
URL der Erstveröffentlichung: https://doi.org/10.1016/j.bone.2018.11.003
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-422463
hdl:20.500.11880/37932
http://dx.doi.org/10.22028/D291-42246
ISSN: 8756-3282
Datum des Eintrags: 24-Jun-2024
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Anatomie und Zellbiologie
Professur: M - Prof. Dr. Gabriela Krasteva-Christ
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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