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doi:10.22028/D291-42234
Titel: | A Quantitative Analysis of Cellular Lipid Compositions During Acute Proteotoxic ER Stress Reveals Specificity in the Production of Asymmetric Lipids |
VerfasserIn: | Reinhard, John Mattes, Carsten Väth, Kristina Radanović, Toni Surma, Michal A. Klose, Christian Ernst, Robert |
Sprache: | Englisch |
Titel: | Frontiers in Cell and Developmental Biology |
Bandnummer: | 8 |
Verlag/Plattform: | Frontiers |
Erscheinungsjahr: | 2020 |
Freie Schlagwörter: | UPR Ire1 lipid bilayer stress proteotoxic stress lipidomics DTT tunicamycin asymmetric lipids |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | The unfolded protein response (UPR) is central to endoplasmic reticulum (ER) homeostasis by controlling its size and protein folding capacity. When activated by unfolded proteins in the ER-lumen or aberrant lipid compositions, the UPR adjusts the expression of hundreds of target genes to counteract ER stress. The proteotoxic drugs dithiothreitol (DTT) and tunicamycin (TM) are commonly used to induce misfolding of proteins in the ER and to study the UPR. However, their potential impact on the cellular lipid composition has never been systematically addressed. Here, we report the quantitative, cellular lipid composition of Saccharomyces cerevisiae during acute, proteotoxic stress in both rich and synthetic media. We show that DTT causes rapid remodeling of the lipidome when used in rich medium at growthinhibitory concentrations, while TM has only a marginal impact on the lipidome under our conditions of cultivation. We formulate recommendations on how to study UPR activation by proteotoxic stress without interferences from a perturbed lipid metabolism. Furthermore, our data suggest an intricate connection between the cellular growth rate, the abundance of the ER, and the metabolism of fatty acids. We show that Saccharomyces cerevisiae can produce asymmetric lipids with two saturated fatty acyl chains differing substantially in length. These observations indicate that the pairing of saturated fatty acyl chains is tightly controlled and suggest an evolutionary conservation of asymmetric lipids and their biosynthetic machineries. |
DOI der Erstveröffentlichung: | 10.3389/fcell.2020.00756 |
URL der Erstveröffentlichung: | https://doi.org/10.3389/fcell.2020.00756 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-422341 hdl:20.500.11880/37919 http://dx.doi.org/10.22028/D291-42234 |
ISSN: | 2296-634X |
Datum des Eintrags: | 21-Jun-2024 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplementary Material |
In Beziehung stehendes Objekt: | https://www.frontiersin.org/articles/file/downloadfile/559605_supplementary-materials_datasheets_1_pdf/octet-stream/Data%20Sheet%201.PDF/1/559605?isPublishedV2=False https://www.frontiersin.org/articles/file/downloadfile/559605_supplementary-materials_datasheets_2_xlsx/octet-stream/Data%20Sheet%202.xlsx/2/559605?isPublishedV2=False |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Medizinische Biochemie und Molekularbiologie |
Professur: | M - Prof. Dr. Robert Ernst |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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fcell-08-00756.pdf | 4,18 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons