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Titel: On the assessment of the stability of vitrified cryo-media by differential scanning calorimetry: A new tool for biobanks to derive standard operating procedures for storage, access and transport
VerfasserIn: Kreiner, Asger UdsID
Stracke, Frank
Zimmermann, Heiko
Sprache: Englisch
In:
Titel: Cryobiology
Bandnummer: 89
Seiten: 26-34
Verlag/Plattform: Elsevier
Erscheinungsjahr: 2019
Freie Schlagwörter: Devitrification
Glass stability
Johnson-Mehl-Avrami-Kolmogorov model
Differential scanning calorimetry
Dimethylsulfoxide
Ethylene glycol
Vitrification
Cryopreservation
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: When a vitrified sample is heated over the glass transition temperature it may start to devitrify endangering the sample. The ability to estimate the stability of the vitrified state can help in the development of new vitrification media as well as handling procedures. By employing differential scanning calorimetry, we can measure the ice crystallization rate in a vitrified sample and thus study the devitrification kinetics. Using this technique, we have studied samples comprised of PBS with cryoprotective additives (CPA) as dimethylsulfoxide (Me2SO), ethylene glycol (EG) and mixtures thereof, regarding the dependence of the devitrification kinetics on the CPA con centration. We found that already small concentration changes lead to significant changes in the devitrification times. Changing the CPA concentration by 4 wt% changed the devitrification time with a factor of 342 and 271 for Me2SO and EG, respectively. Concentration changes in EG/Me2SO mixtures was found to have a smaller impact on the devitrification kinetics compared to the pure CPA samples. Our data suggest that these significant increases in the devitrification times are primarily due to a relation between nucleation rates and the CPA concentration. Finally, we investigated an established vitrification medium used to preserve human embryonic stem cells. This medium was found to have the poorest glass stability in this study and reflects the tradeoff between stability and biocompatibility. The present work finally provides a tool to evaluate handling and storage procedures when employing vitrification as a cryopreservation method and underlines the importance of these.
DOI der Erstveröffentlichung: 10.1016/j.cryobiol.2019.06.002
URL der Erstveröffentlichung: https://doi.org/10.1016/j.cryobiol.2019.06.002
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-419487
hdl:20.500.11880/37537
http://dx.doi.org/10.22028/D291-41948
ISSN: 0011-2240
Datum des Eintrags: 26-Apr-2024
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Prof. Dr. Heiko Zimmermann
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes



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