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doi:10.22028/D291-41907
Titel: | PIEZO1-mediated mechanosensing governs NK-cell killing efficiency and infiltration in three-dimensional matrices |
VerfasserIn: | Yanamandra, Archana K. Zhang, Jingnan Montalvo, Galia Zhou, Xiangda Biedenweg, Doreen Zhao, Renping Sharma, Shulagna Hoth, Markus Lautenschläger, Franziska Otto, Oliver del Campo, Aránzazu Qu, Bin |
Sprache: | Englisch |
Titel: | European Journal of Immunology |
Bandnummer: | 54 |
Heft: | 3 |
Verlag/Plattform: | Wiley |
Erscheinungsjahr: | 2024 |
Freie Schlagwörter: | 3D matrices Killing efficiency Mechanosensing NK cells PIEZO1 |
DDC-Sachgruppe: | 500 Naturwissenschaften |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Natural killer (NK) cells play a vital role in eliminating tumorigenic cells. Efficient locating and killing of target cells in complex three-dimensional (3D) environments are critical for their functions under physiological conditions. However, the role of mechanosensing in regulating NK-cell killing efficiency in physiologically relevant scenarios is poorly understood. Here, we report that the responsiveness of NK cells is regulated by tumor cell stiffness. NK-cell killing efficiency in 3D is impaired against softened tumor cells, whereas it is enhanced against stiffened tumor cells. Notably, the durations required for NK-cell killing and detachment are significantly shortened for stiffened tumor cells. Furthermore, we have identified PIEZO1 as the predominantly expressed mechanosensitive ion channel among the examined candidates in NK cells. Perturbation of PIEZO1 abolishes stiffness-dependent NK-cell responsiveness, significantly impairs the killing efficiency of NK cells in 3D, and substantially reduces NK-cell infiltration into 3D collagen matrices. Conversely, PIEZO1 activation enhances NK killing efficiency as well as infiltration. In conclusion, our findings demonstrate that PIEZO1-mediated mechanosensing is crucial for NK killing functions, highlighting the role of mechanosensing in NK-cell killing efficiency under 3D physiological conditions and the influence of environmental physical cues on NK-cell functions. |
DOI der Erstveröffentlichung: | 10.1002/eji.202350693 |
URL der Erstveröffentlichung: | https://doi.org/10.1002/eji.202350693 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-419076 hdl:20.500.11880/37487 http://dx.doi.org/10.22028/D291-41907 |
ISSN: | 1521-4141 0014-2980 |
Datum des Eintrags: | 16-Apr-2024 |
Bezeichnung des in Beziehung stehenden Objekts: | Supporting Information |
In Beziehung stehendes Objekt: | https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Feji.202350693&file=eji5670-sup-0001-Movie1.mp4 https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Feji.202350693&file=eji5670-sup-0002-Movie2.mp4 https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Feji.202350693&file=eji5670-sup-0003-Movie3.mp4 https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Feji.202350693&file=eji5670-sup-0004-Movie4.mp4 https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Feji.202350693&file=eji5670-sup-0005-Movie5.mp4 https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Feji.202350693&file=eji5670-sup-0006-Movie6.mp4 https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Feji.202350693&file=eji5670-sup-0007-SuppMat.pdf |
Fakultät: | M - Medizinische Fakultät NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | M - Biophysik NT - Chemie NT - Physik |
Professur: | M - Prof. Dr. Markus Hoth NT - Prof. Dr. Aránzazu del Campo NT - Jun.-Prof. Dr. Franziska Lautenschläger |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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Eur J Immunol - 2024 - Yanamandra - PIEZO1‐mediated mechanosensing governs NK‐cell killing efficiency and infiltration in.pdf | 2,5 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons