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Titel: Engineered living materials for the conversion of a low-cost food-grade precursor to a high-value flavonoid
VerfasserIn: Riedel, Florian
Bartolomé, Maria Puertas
Enrico, Lara Luana Teruel
Fink-Straube, Claudia
Duong, Cao Nguyen
Gherlone, Fabio
Huang, Ying
Valiante, Vito
del Campo, Aránzazu
Sankaran, Shrikrishnan
Sprache: Englisch
Titel: Frontiers in Bioengineering and Biotechnology
Bandnummer: 11
Verlag/Plattform: Frontiers
Erscheinungsjahr: 2023
Freie Schlagwörter: engineered-living-materials (ELMs)
flavonoid
pinocembrin
PVA hydrogel
E. coli Nissle 1917
probiotic
enzyme catalysis
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Microbial biofactories allow the upscaled production of high-value compounds in biotechnological processes. This is particularly advantageous for compounds like flavonoids that promote better health through their antioxidant, anti-bacterial, anti-cancer and other beneficial effects but are produced in small quantities in their natural plant-based hosts. Bacteria like E. coli have been genetically modified with enzyme cascades to produce flavonoids like naringenin and pinocembrin from coumaric or cinnamic acid. Despite advancements in yield optimization, the production of these compounds still involves high costs associated with their biosynthesis, purification, storage and transport. An alternative production strategy could involve the direct delivery of the microbial biofactories to the body. In such a strategy, ensuring biocontainment of the engineered microbes in the body and controlling production rates are major challenges. In this study, these two aspects are addressed by developing engineered living materials (ELMs) consisting of probiotic microbial biofactories encapsulated in biocompatible hydrogels. Engineered probiotic E. coli Nissle 1917 able to efficiently convert cinnamic acid into pinocembrin were encapsulated in poly(vinyl alcohol)-based hydrogels. The biofactories are contained in the hydrogels for a month and remain metabolically active during this time. Control over production levels is achieved by the containment inside the material, which regulates bacteria growth, and by the amount of cinnamic acid in the medium.
DOI der Erstveröffentlichung: 10.3389/fbioe.2023.1278062
URL der Erstveröffentlichung: https://doi.org/10.3389/fbioe.2023.1278062
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-417481
hdl:20.500.11880/37363
http://dx.doi.org/10.22028/D291-41748
ISSN: 2296-4185
Datum des Eintrags: 13-Mär-2024
Bezeichnung des in Beziehung stehenden Objekts: Supplementary material
In Beziehung stehendes Objekt: https://ndownloader.figstatic.com/files/43317168
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Chemie
Professur: NT - Prof. Dr. Aránzazu del Campo
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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