Please use this identifier to cite or link to this item: doi:10.22028/D291-41545
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Title: Short-Term Effect of Rose Bengal Photodynamic Therapy (RB-PDT) on Collagen I, Collagen V, NF-κB, LOX, TGF-β and IL-6 Expression of Human Corneal Fibroblasts, In Vitro
Author(s): Chai, Ning
Stachon, Tanja
Berger, Tim
Li, Zhen
Seitz, Berthold
Langenbucher, Achim
Szentmáry, Nóra
Language: English
Title: Current Eye Research
Volume: 49
Issue: 2
Pages: 150-157
Publisher/Platform: Taylor & Francis
Year of Publication: 2024
Free key words: Human corneal fibroblasts
photodynamic therapy
Rose Bengal
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Abstract Purpose To investigate collagen I, collagen V, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), lysyl oxidase (LOX), transforming growth factor β1 (TGF-β1) and interleukin-6 (IL-6) expression in healthy and keratoconus human corneal fibroblasts (HCFs and KC-HCFs), 24 h after Rose Bengal photodynamic therapy (RB-PDT). Methods HCFs were isolated from healthy human corneal donors (n = 5) and KC-HCFs from elective penetrating keratoplasties (n = 5). Both cell cultures underwent RB-PDT (0.001% RB concentration, 0.17 J/cm2 fluence) and 24 h later collagen I, collagen V, NF-κB, LOX, TGF-β1 and IL-6 mRNA and protein expression have been determined using qPCR and Western blot, IL-6 concentration in the cell culture supernatant by ELISA. Results TGF-β1 mRNA expression was significantly lower (p = 0.02) and IL-6 mRNA expression was significantly higher in RB-PDT treated HCFs (p = 0.01), than in HCF controls. COL1A1, COL5A1 and TGF-β1 mRNA expression was significantly lower (p = 0.04; p = 0.02 and p = 0.003) and IL-6 mRNA expression was significantly higher (p = 0.02) in treated KC-HCFs, than in KC-HCF controls. TGF-β1 protein expression in treated HCFs was significantly higher than in HCF controls (p = 0.04). IL-6 protein concentration in the HCF and KC-HCF culture supernatant after RB-PDT was significantly higher than in controls (p = 0.02; p = 0.01). No other analyzed mRNA and protein expression differed significantly between the RB-PDT treated and untreated groups. Conclusions Our study demonstrates that RB-PDT reduces collagen I, collagen V and TGF-β1 mRNA expression, while increasing IL-6 mRNA and protein expression in KC-HCFs. In HCFs, RB-PDT increases TGF-β1 and IL-6 protein level after 24 h.
DOI of the first publication: 10.1080/02713683.2023.2276057
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-415456
ISSN: 1460-2202
Date of registration: 2-Feb-2024
Faculty: M - Medizinische Fakultät
Department: M - Augenheilkunde
Professorship: M - Univ.-Prof. Dr. Dipl.-Ing. Achim Langenbucher
M - Prof. Dr. Berthold Seitz
M - Prof. Dr. med. Nóra Szentmáry
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