Please use this identifier to cite or link to this item: doi:10.22028/D291-41510
Title: Calcium Electroporation versus Electrochemotherapy with Bleomycin in an In Vivo CAM-Based Uveal Melanoma Xenograft Model
Author(s): Tsimpaki, Theodora
Anastasova, Ralitsa
Liu, Hongtao
Seitz, Berthold
Bechrakis, Nikolaos E.
Berchner-Pfannschmidt, Utta
Kraemer, Miriam M.
Fiorentzis, Miltiadis
Language: English
Title: International Journal of Molecular Sciences
Volume: 25
Issue: 2
Publisher/Platform: MDPI
Year of Publication: 2024
Free key words: uveal melanoma
calcium electroporation
chick chorioallantois membrane assay
uveal melanoma xenografts
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Despite recent advancements in the diagnosis and treatment of uveal melanoma (UM), its metastatic rate remains high and is accompanied by a highly dismal prognosis, constituting an unmet need for the development of novel adjuvant therapeutic strategies. We established an in vivo chick chorioallantoic membrane (CAM)-based UM xenograft model from UPMD2 and UPMM3 cell lines to examine its feasibility for the improvement of selection of drug candidates. The efficacy of calcium electroporation (CaEP) with 5 or 10 mM calcium chloride (Ca) and electrochemotherapy (ECT) with 1 or 2.5 µg/mL bleomycin in comparison to monotherapy with the tested drug or electroporation (EP) alone was investigated on the generated UM tumors. CaEP and ECT showed a similar reduction of proliferation and melanocytic expansion with a dose-dependent effect for bleomycin, whereas CaEP induced a significant increase of the apoptosis and a reduction of vascularization with varying sensitivity for the two xenograft types. Our in vivo results suggest that CaEP and ECT may facilitate the adequate local tumor control and contribute to the preservation of the bulbus, potentially opening new horizons in the adjuvant treatment of advanced UM.
DOI of the first publication: 10.3390/ijms25020938
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-415100
ISSN: 1422-0067
Date of registration: 29-Jan-2024
Faculty: M - Medizinische Fakultät
Department: M - Augenheilkunde
Professorship: M - Prof. Dr. Berthold Seitz
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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