Please use this identifier to cite or link to this item: doi:10.22028/D291-41508
Title: Nanofat Accelerates and Improves the Vascularization, Lymphatic Drainage and Healing of Full-Thickness Murine Skin Wounds
Author(s): Limido, Ettore
Weinzierl, Andrea
Ampofo, Emmanuel
Harder, Yves
Menger, Michael D.
Laschke, Matthias W.
Language: English
Title: International Journal of Molecular Sciences
Volume: 25
Issue: 2
Publisher/Platform: MDPI
Year of Publication: 2024
Free key words: wound healing
platelet-rich plasma
lymph vessels
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: The treatment of wounds using the body’s own resources is a promising approach to support the physiological regenerative process. To advance this concept, we evaluated the effect of nanofat (NF) on wound healing. For this purpose, full-thickness skin defects were created in dorsal skinfold chambers of wild-type mice. These defects were filled with NF generated from the inguinal subcutaneous adipose tissue of green fluorescent protein (GFP)+ donor mice, which was stabilized using platelet-rich plasma (PRP). Empty wounds and wounds solely filled with PRP served as controls. Wound closure, vascularization and formation of granulation tissue were repeatedly analyzed using stereomicroscopy, intravital fluorescence microscopy, histology and immunohistochemistry over an observation period of 14 days. PRP + NF-treated wounds exhibited accelerated vascularization and wound closure when compared to controls. This was primarily due to the fact that the grafted NF contained a substantial fraction of viable GFP+ vascular and lymph vessel fragments, which interconnected with the GFP− vessels of the host tissue. Moreover, the switch from inflammatory M1- to regenerative M2-polarized macrophages was promoted in PRP + NF-treated wounds. These findings indicate that NF markedly accelerates and improves the wound healing process and, thus, represents a promising autologous product for future wound management.
DOI of the first publication: 10.3390/ijms25020851
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-415089
ISSN: 1422-0067
Date of registration: 29-Jan-2024
Faculty: M - Medizinische Fakultät
Department: M - Chirurgie
Professorship: M - Prof. Dr. Michael D. Menger
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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