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doi:10.22028/D291-41484
Titel: | Stable tug-of-war between kinesin-1 and cytoplasmic dynein upon different ATP and roadblock concentrations |
VerfasserIn: | Monzon, Gina A. Scharrel, Lara DSouza, Ashwin Henrichs, Verena Santen, Ludger Diez, Stefan |
Sprache: | Englisch |
Titel: | Journal of Cell Science |
Bandnummer: | 133 |
Heft: | 22 |
Verlag/Plattform: | Company of Biologists Limited |
Erscheinungsjahr: | 2020 |
Freie Schlagwörter: | Kinesin Dynein ATP concentration Roadblocks Gliding motility assay Stochastic modeling Tug-of-war |
DDC-Sachgruppe: | 500 Naturwissenschaften |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | The maintenance of intracellular processes, like organelle transport and cell division, depend on bidirectional movement along microtubules. These processes typically require kinesin and dynein motor proteins, which move with opposite directionality. Because both types of motors are often simultaneously bound to the cargo, regulatory mechanisms are required to ensure controlled directional transport. Recently, it has been shown that parameters like mechanical motor activation, ATP concentration and roadblocks on the microtubule surface differentially influence the activity of kinesin and dynein motors in distinct manners. However, how these parameters affect bidirectional transport systems has not been studied. Here, we investigate the regulatory influence of these three parameters using in vitro gliding motility assays and stochastic simulations. We find that the number of active kinesin and dynein motors determines the transport direction and velocity, but that variations in ATP concentration and roadblock density have no significant effect. Thus, factors influencing the force balance between opposite motors appear to be important, whereas the detailed stepping kinetics and bypassing capabilities of the motors only have a small effect. |
DOI der Erstveröffentlichung: | 10.1242/jcs.249938 |
URL der Erstveröffentlichung: | https://doi.org/10.1242/jcs.249938 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-414849 hdl:20.500.11880/37164 http://dx.doi.org/10.22028/D291-41484 |
ISSN: | 1477-9137 0021-9533 |
Datum des Eintrags: | 23-Jan-2024 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplementary information |
In Beziehung stehendes Objekt: | https://cob.silverchair-cdn.com/cob/content_public/journal/jcs/133/22/10.1242_jcs.249938/3/jcs249938supp.pdf?Expires=1708444603&Signature=gPpKda7~I0FcwNAJxq6wvAZPokb0-EuDx2TtaD8~47WeSefZ3H6kINiami~djUmTOck8NguFPVks~vsk9SVfX40oelWgsj9vWNot7~PNb7uEt-6FfD6C6ybuvK77ksWjjKhfdyF7~j7019mas48zPjcb87ugyiN0CLrt2H8UZYxkkarLq7GFY0VQphsTI6QRA3ul-XmB0trQ~XVPw9W4cnD6s9dAYiiY2qrBDEbLneC9g1jtolEaH3QoVSkI1taYnDvPfPocH2C5Ocq9WStmtGNFyTgG4VnBMc3Q4JQoXuE1HrmOQ1LRtzuRuT1~1pAUlNu9KN669U579Ht9BcXARw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Physik |
Professur: | NT - Prof. Dr. Ludger Santen |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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