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Titel: Stable tug-of-war between kinesin-1 and cytoplasmic dynein upon different ATP and roadblock concentrations
VerfasserIn: Monzon, Gina A.
Scharrel, Lara
DSouza, Ashwin
Henrichs, Verena
Santen, Ludger
Diez, Stefan
Sprache: Englisch
Titel: Journal of Cell Science
Bandnummer: 133
Heft: 22
Verlag/Plattform: Company of Biologists Limited
Erscheinungsjahr: 2020
Freie Schlagwörter: Kinesin
Dynein
ATP concentration
Roadblocks
Gliding motility assay
Stochastic modeling
Tug-of-war
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: The maintenance of intracellular processes, like organelle transport and cell division, depend on bidirectional movement along microtubules. These processes typically require kinesin and dynein motor proteins, which move with opposite directionality. Because both types of motors are often simultaneously bound to the cargo, regulatory mechanisms are required to ensure controlled directional transport. Recently, it has been shown that parameters like mechanical motor activation, ATP concentration and roadblocks on the microtubule surface differentially influence the activity of kinesin and dynein motors in distinct manners. However, how these parameters affect bidirectional transport systems has not been studied. Here, we investigate the regulatory influence of these three parameters using in vitro gliding motility assays and stochastic simulations. We find that the number of active kinesin and dynein motors determines the transport direction and velocity, but that variations in ATP concentration and roadblock density have no significant effect. Thus, factors influencing the force balance between opposite motors appear to be important, whereas the detailed stepping kinetics and bypassing capabilities of the motors only have a small effect.
DOI der Erstveröffentlichung: 10.1242/jcs.249938
URL der Erstveröffentlichung: https://doi.org/10.1242/jcs.249938
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-414849
hdl:20.500.11880/37164
http://dx.doi.org/10.22028/D291-41484
ISSN: 1477-9137
0021-9533
Datum des Eintrags: 23-Jan-2024
Bezeichnung des in Beziehung stehenden Objekts: Supplementary information
In Beziehung stehendes Objekt: https://cob.silverchair-cdn.com/cob/content_public/journal/jcs/133/22/10.1242_jcs.249938/3/jcs249938supp.pdf?Expires=1708444603&Signature=gPpKda7~I0FcwNAJxq6wvAZPokb0-EuDx2TtaD8~47WeSefZ3H6kINiami~djUmTOck8NguFPVks~vsk9SVfX40oelWgsj9vWNot7~PNb7uEt-6FfD6C6ybuvK77ksWjjKhfdyF7~j7019mas48zPjcb87ugyiN0CLrt2H8UZYxkkarLq7GFY0VQphsTI6QRA3ul-XmB0trQ~XVPw9W4cnD6s9dAYiiY2qrBDEbLneC9g1jtolEaH3QoVSkI1taYnDvPfPocH2C5Ocq9WStmtGNFyTgG4VnBMc3Q4JQoXuE1HrmOQ1LRtzuRuT1~1pAUlNu9KN669U579Ht9BcXARw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Physik
Professur: NT - Prof. Dr. Ludger Santen
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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