Please use this identifier to cite or link to this item: doi:10.22028/D291-41122
Title: Inflammatory Mediators in Tracheal Aspirates of Preterm Infants Participating in a Randomized Trial of Inhaled Nitric Oxide
Author(s): Laube, Mandy
Amann, Elena
Uhlig, Ulrike
Yang, Yang
Fuchs, Hans W.
Zemlin, Michael
Mercier, Jean-Christophe
Maier, Rolf F
Hummler, Helmut D.
Uhlig, Stefan
Thome, Ulrich H.
Language: English
Title: PloS one
Volume: 12
Issue: 1
Publisher/Platform: Plos
Year of Publication: 2017
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Background Ventilated preterm infants frequently develop bronchopulmonary dysplasia (BPD) which is associated with elevated inflammatory mediators in their tracheal aspirates (TA). In animal models of BPD, inhaled nitric oxide (iNO) has been shown to reduce lung inflammation, but data for human preterm infants is missing. Methods Within a European multicenter trial of NO inhalation for preterm infants to prevent BPD (EUNO), TA was collected to determine the effects of iNO on pulmonary inflammation. TA was collected from 43 premature infants randomly assigned to receive either iNO or placebo gas (birth weight 530–1230 g, median 800 g, gestational age 24 to 28 2/7 weeks, median 26 weeks). Interleukin (IL)-1β, IL-6, IL-8, transforming growth factor (TGF)-β1, interferon γinduced protein 10 (IP-10), macrophage inflammatory protein (MIP)-1α, acid sphingomyelinase (ASM), neuropeptide Y and leukotriene B4 were measured in serial TA samples from postnatal day 2 to 14. Furthermore, TA levels of nitrotyrosine and nitrite were determined under iNO therapy. Results The TA levels of IP-10, IL-6, IL-8, MIP-1α, IL-1β, ASM and albumin increased with advancing postnatal age in critically ill preterm infants, whereas nitrotyrosine TA levels declined in both, iNO-treated and placebo-treated infants. The iNO treatment generally increased nitrite TA levels, whereas nitrotyrosine TA levels were not affected by iNO treatment. Furthermore, iNO treatment transiently reduced early inflammatory and fibrotic markers associated with BPD development including TGF-β1, IP-10 and IL-8, but induced a delayed increase of ASM TA levels. Conclusion Treatment with iNO may have played a role in reducing several inflammatory and fibrotic mediators in TA of preterm infants compared to placebo-treated infants. However, survival without BPD was not affected in the main EUNO trial.
DOI of the first publication: 10.1371/journal.pone.0169352
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-411229
ISSN: 1932-6203
Date of registration: 20-Nov-2023
Faculty: M - Medizinische Fakultät
Department: M - Pädiatrie
Professorship: M - Prof. Dr. Michael Zemlin
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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