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Titel: Investigation of CSF outflow in mice by in vivo, dynamic magnetic resonance imaging
VerfasserIn: Krämer, Jonas Uwe
Sprache: Englisch
Erscheinungsjahr: 2022
Erscheinungsort: Homburg/Saar
DDC-Sachgruppe: 000 Allgemeines, Wissenschaft
610 Medizin, Gesundheit
Dokumenttyp: Dissertation
Abstract: The anatomical routes for the clearance of cerebrospinal fluid (CSF) remain incompletely understood. However, recent evidence has given strong support for routes leading to lymphatic vessels. A current debate centers upon the routes through which CSF can access lymphatics, with evidence emerging for either direct routes to meningeal lymphatics or along cranial nerves to reach lymphatics outside the skull. Here, a method was established to infuse contrast agent into the ventricles using indwelling cannulae during imaging of mice at 2 and 12 months of age by magnetic resonance imaging. As expected, a substantial decline in overall CSF turnover was found with aging. Quantifications demonstrated that the bulk of the contrast agent flowed from the ventricles to the subarachnoid space in the basal cisterns. Comparatively little contrast agent signal was found at the dorsal aspect of the skull. The imaging dynamics from the 2 cohorts revealed that the contrast agent was cleared from the cranium through the cribriform plate to the nasopharyngeal lymphatics. On decalcified sections, we confirmed that fluorescently labeled ovalbumin drained through the cribriform plate and could be found within lymphatics surrounding the nasopharynx. In conclusion, routes leading to nasopharyngeal lymphatics appear to be a major efflux pathway for cranial CSF.
DOI der Erstveröffentlichung: 2022
URL der Erstveröffentlichung: https://doi.org/10.1172/jci
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-407304
hdl:20.500.11880/36709
http://dx.doi.org/10.22028/D291-40730
Erstgutachter: Faßbender, Klaus
Tag der mündlichen Prüfung: 17-Okt-2023
Datum des Eintrags: 27-Okt-2023
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Neurologie und Psychiatrie
Professur: M - Prof. Dr. Klaus Faßbender
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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