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doi:10.22028/D291-40648
Titel: | Remodeling of yeast vacuole membrane lipidomes from the log (one phase) to stationary stage (two phases) |
VerfasserIn: | Reinhard, John Leveille, Chantelle L. Cornell, Caitlin E. Merz, Alexey J. Klose, Christian Ernst, Robert Keller, Sarah L. |
Sprache: | Englisch |
Titel: | Biophysical Journal |
Bandnummer: | 122 |
Heft: | 6 |
Seiten: | 1043-1057 |
Verlag/Plattform: | Elsevier |
Erscheinungsjahr: | 2023 |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Upon nutrient limitation, budding yeast of Saccharomyces cerevisiae shift from fast growth (the log stage) to quiescence (the stationary stage). This shift is accompanied by liquid-liquid phase separation in the membrane of the vacuole, an endosomal organelle. Recent work indicates that the resulting micrometer-scale domains in vacuole membranes enable yeast to survive periods of stress. An outstanding question is which molecular changes might cause this membrane phase separation. Here, we conduct lipidomics of vacuole membranes in both the log and stationary stages. Isolation of pure vacuole membranes is challenging in the stationary stage, when lipid droplets are in close contact with vacuoles. Immuno-isolation has previously been shown to successfully purify log-stage vacuole membranes with high organelle specificity, but it was not previously possible to immuno-isolate stationary-stage vacuole membranes. Here, we develop Mam3 as a bait protein for vacuole immuno-isolation, and demonstrate low contamination by non-vacuolar membranes. We find that stationary-stage vacuole membranes contain surprisingly high fractions of phosphatidylcholine lipids ( 40%), roughly twice as much as log-stage membranes. Moreover, in the stationary stage, these lipids have higher melting temperatures, due to longer and more saturated acyl chains. Another surprise is that no significant change in sterol content is observed. These lipidomic changes, which are largely reflected on the whole-cell level, fit within the predominant view that phase separation in membranes requires at least three types of molecules to be present: lipids with high melting temperatures, lipids with low melting temperatures, and sterols. |
DOI der Erstveröffentlichung: | 10.1016/j.bpj.2023.01.009 |
URL der Erstveröffentlichung: | https://doi.org/10.1016/j.bpj.2023.01.009 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-406480 hdl:20.500.11880/36518 http://dx.doi.org/10.22028/D291-40648 |
ISSN: | 0006-3495 |
Datum des Eintrags: | 28-Sep-2023 |
Bezeichnung des in Beziehung stehenden Objekts: | Supporting material |
In Beziehung stehendes Objekt: | https://ars.els-cdn.com/content/image/1-s2.0-S0006349523000255-mmc1.pdf https://ars.els-cdn.com/content/image/1-s2.0-S0006349523000255-mmc2.xlsx https://ars.els-cdn.com/content/image/1-s2.0-S0006349523000255-mmc3.pdf |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Medizinische Biochemie und Molekularbiologie |
Professur: | M - Prof. Dr. Robert Ernst |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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1-s2.0-S0006349523000255-main.pdf | 1,56 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons