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doi:10.22028/D291-40348
Titel: | Light‐Regulated Pro‐Angiogenic Engineered Living Materials |
VerfasserIn: | Dhakane, Priyanka Tadimarri, Varun Sai Sankaran, Shrikrishnan |
Sprache: | Englisch |
Titel: | Advanced Functional Materials |
Bandnummer: | 33 |
Heft: | 31 |
Verlag/Plattform: | Wiley |
Erscheinungsjahr: | 2023 |
Freie Schlagwörter: | angiogenesis bacteria engineered living materials growth factor optogenetics |
DDC-Sachgruppe: | 500 Naturwissenschaften |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Regenerative medicine aims to restore damaged cells, tissues, and organs, for which growth factors are vital to stimulate regenerative cellular transformations. Major advances have been made in growth factor engineering and delivery like the development of robust peptidomimetics and controlled release matrices. However, their clinical applicability remains limited due to their poor stability in the body and need for careful regulation of their local concentration to avoid unwanted side-effects. In this study, a strategy to overcome these limitations is explored using engineered living materials (ELMs), which contain live microorganisms that can be programmed with stimuliresponsive functionalities. Specifically, the development of an ELM that releases a pro-angiogenic protein in a light-regulated manner is described. This is achieved by optogenetically engineering bacteria to synthesize and secrete a vascular endothelial growth factor peptidomimetic (QK) linked to a collagen-binding domain. The bacteria are securely encapsulated in bilayer hydrogel constructs that support bacterial functionality but prevent their escape from the ELM. In situ control over the release profiles of the proangiogenic protein using light is demonstrated. Finally, it is shown that the released protein is able to bind collagen and promote angiogenic network formation among vascular endothelial cells, indicating the regenerative potential of these ELMs. |
DOI der Erstveröffentlichung: | 10.1002/adfm.202212695 |
URL der Erstveröffentlichung: | https://doi.org/10.1002/adfm.202212695 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-403485 hdl:20.500.11880/36368 http://dx.doi.org/10.22028/D291-40348 |
ISSN: | 1616-3028 |
Datum des Eintrags: | 4-Sep-2023 |
Bezeichnung des in Beziehung stehenden Objekts: | Supporting Information |
In Beziehung stehendes Objekt: | https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadfm.202212695&file=adfm202212695-sup-0001-SuppMat.pdf |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Materialwissenschaft und Werkstofftechnik |
Professur: | NT - Prof. Dr. Aránzazu del Campo |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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Adv Funct Materials - 2023 - Dhakane.pdf | 2,71 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons