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Titel: Light‐Regulated Pro‐Angiogenic Engineered Living Materials
VerfasserIn: Dhakane, Priyanka
Tadimarri, Varun Sai
Sankaran, Shrikrishnan
Sprache: Englisch
Titel: Advanced Functional Materials
Bandnummer: 33
Heft: 31
Verlag/Plattform: Wiley
Erscheinungsjahr: 2023
Freie Schlagwörter: angiogenesis
bacteria
engineered living materials
growth factor
optogenetics
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Regenerative medicine aims to restore damaged cells, tissues, and organs, for which growth factors are vital to stimulate regenerative cellular transformations. Major advances have been made in growth factor engineering and delivery like the development of robust peptidomimetics and controlled release matrices. However, their clinical applicability remains limited due to their poor stability in the body and need for careful regulation of their local concentration to avoid unwanted side-effects. In this study, a strategy to overcome these limitations is explored using engineered living materials (ELMs), which contain live microorganisms that can be programmed with stimuliresponsive functionalities. Specifically, the development of an ELM that releases a pro-angiogenic protein in a light-regulated manner is described. This is achieved by optogenetically engineering bacteria to synthesize and secrete a vascular endothelial growth factor peptidomimetic (QK) linked to a collagen-binding domain. The bacteria are securely encapsulated in bilayer hydrogel constructs that support bacterial functionality but prevent their escape from the ELM. In situ control over the release profiles of the proangiogenic protein using light is demonstrated. Finally, it is shown that the released protein is able to bind collagen and promote angiogenic network formation among vascular endothelial cells, indicating the regenerative potential of these ELMs.
DOI der Erstveröffentlichung: 10.1002/adfm.202212695
URL der Erstveröffentlichung: https://doi.org/10.1002/adfm.202212695
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-403485
hdl:20.500.11880/36368
http://dx.doi.org/10.22028/D291-40348
ISSN: 1616-3028
Datum des Eintrags: 4-Sep-2023
Bezeichnung des in Beziehung stehenden Objekts: Supporting Information
In Beziehung stehendes Objekt: https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadfm.202212695&file=adfm202212695-sup-0001-SuppMat.pdf
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Materialwissenschaft und Werkstofftechnik
Professur: NT - Prof. Dr. Aránzazu del Campo
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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