Please use this identifier to cite or link to this item: doi:10.22028/D291-40343
Title: Protocol to identify amino acids bound to tRNA by aminoacylation using mass spectrometry
Author(s): Finkler, Marc
Brandt, Joshua
Boutfol, Timothée
Grimm, Florent
Hartz, Philip
Ott, Albrecht
Language: English
Title: STAR Protocols
Volume: 4
Issue: 3
Publisher/Platform: Elsevier
Year of Publication: 2023
Free key words: Gene Expression
Protein Expression and Purification
Biotechnology and Bioengineering
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: tRNA-bound amino acids often need to be identified, for instance, in cases where different amino acids compete for binding to the same tRNA. Here, we present a mass-spectrometry-based protocol to determine the amino acids bound to tRNA by aminoacylation. We detail how to perform the aminoacylation reaction, the preparation of the aminoacyl-tRNA for measurement, and the mass spectrometric analysis. We use arginine acylation as an example; however, this protocol can be applied to any other amino acid.
DOI of the first publication: 10.1016/j.xpro.2023.102504
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-403435
ISSN: 2666-1667
Date of registration: 21-Aug-2023
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Prof. Dr. Albrecht Ott
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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