Please use this identifier to cite or link to this item: doi:10.22028/D291-40201
Title: Plasma Membrane Calcium ATPase Regulates Stoichiometry of CD4+ T-Cell Compartments
Author(s): Merino-Wong, Maylin
Niemeyer, Barbara A.
Alansary, Dalia
Language: English
Title: Frontiers in Immunology
Volume: 12
Publisher/Platform: Frontiers
Year of Publication: 2021
Free key words: Plasma Membrane Calcium ATPase
Yin Yang 1
CD4+ compartments
calcium signaling
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Immune responses involve mobilization of T cells within naïve and memory compartments. Tightly regulated Ca2+ levels are essential for balanced immune outcomes. How Ca2+ contributes to regulating compartment stoichiometry is unknown. Here, we show that plasma membrane Ca2+ ATPase 4 (PMCA4) is differentially expressed in human CD4+ T compartments yielding distinct store operated Ca2+ entry (SOCE) profiles. Modulation of PMCA4 yielded a more prominent increase of SOCE in memory than in naïve CD4+ T cell. Interestingly, downregulation of PMCA4 reduced the effector compartment fraction and led to accumulation of cells in the naïve compartment. In silico analysis and chromatin immunoprecipitation point towards Ying Yang 1 (YY1) as a transcription factor regulating PMCA4 expression. Analyses of PMCA and YY1 expression patterns following activation and of PMCA promoter activity following downregulation of YY1 highlight repressive role of YY1 on PMCA expression. Our findings show that PMCA4 adapts Ca2+ levels to cellular requirements during effector and quiescent phases and thereby represent a potential target to intervene with the outcome of the immune response.
DOI of the first publication: 10.3389/fimmu.2021.687242
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-402012
ISSN: 1664-3224
Date of registration: 26-Jul-2023
Description of the related object: Supplementary Material
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Biophysik
Professorship: M - Prof. Dr. Barbara Niemeyer-Hoth
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Files for this record:
File Description SizeFormat 
fimmu-12-687242.pdf5,48 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons