Please use this identifier to cite or link to this item: doi:10.22028/D291-40187
Title: Localization of the Priming Factors CAPS1 and CAPS2 in Mouse Sensory Neurons Is Determined by Their N-Termini
Author(s): Staudt, Angelina
Ratai, Olga
Bouzouina, Aicha
Fecher-Trost, Claudia
Shaaban, Ahmed
Bzeih, Hawraa
Horn, Alexander
Shaib, Ali H.
Klose, Margarete
Flockerzi, Veit
Lauterbach, Marcel A.
Rettig, Jens
Becherer, Ute
Language: English
Title: Frontiers in Molecular Neuroscience
Volume: 15
Publisher/Platform: Frontiers
Year of Publication: 2022
Free key words: dorsal root ganglion
cellular localization
intracellular trafficking
active zone
STED microscopy
DDC notations: 500 Science
610 Medicine and health
Publikation type: Journal Article
Abstract: Both paralogs of the calcium-dependent activator protein for secretion (CAPS) are required for exocytosis of synaptic vesicles (SVs) and large dense core vesicles (LDCVs). Despite approximately 80% sequence identity, CAPS1 and CAPS2 have distinct functions in promoting exocytosis of SVs and LDCVs in dorsal root ganglion (DRG) neurons. However, the molecular mechanisms underlying these differences remain enigmatic. In this study, we applied high- and super-resolution imaging techniques to systematically assess the subcellular localization of CAPS paralogs in DRG neurons deficient in both CAPS1 and CAPS2. CAPS1 was found to be more enriched at the synapses. Using – in-depth sequence analysis, we identified a unique CAPS1 N-terminal sequence, which we introduced into CAPS2. This CAPS1/2 chimera reproduced the pre-synaptic localization of CAPS1 and partially rescued synaptic transmission in neurons devoid of CAPS1 and CAPS2. Using immunoprecipitation combined with mass spectrometry, we identified CAPS1-specific interaction partners that could be responsible for its pre-synaptic enrichment. Taken together, these data suggest an important role of the CAPS1-N terminus in the localization of the protein at pre-synapses.
DOI of the first publication: 10.3389/fnmol.2022.674243
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-401879
ISSN: 1662-5099
Date of registration: 25-Jul-2023
Description of the related object: Supplementary Material
Related object:
Faculty: M - Medizinische Fakultät
NT - Naturwissenschaftlich- Technische Fakultät
Department: M - Experimentelle und Klinische Pharmakologie und Toxikologie
M - Physiologie
NT - Chemie
Professorship: M - Prof. Dr. Veit Flockerzi
M - Prof. Dr. Jens Rettig
NT - Prof. Dr. Uli Kazmaier
M - Jun.-Prof. Dr. Marcel Lauterbach
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Files for this record:
File Description SizeFormat 
fnmol-15-674243.pdf11,56 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons