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Titel: Changes in Posttraumatic Brain Edema in Craniectomy-Selective Brain Hypothermia Model Are Associated With Modulation of Aquaporin-4 Level
VerfasserIn: Szczygielski, Jacek
Glameanu, Cosmin
Müller, Andreas
Klotz, Markus
Sippl, Christoph
Hubertus, Vanessa
Schäfer, Karl-Herbert
Mautes, Angelika E.
Schwerdtfeger, Karsten
Oertel, Joachim
Sprache: Englisch
Titel: Frontiers in Neurology
Bandnummer: 9
Verlag/Plattform: Frontiers
Erscheinungsjahr: 2018
Freie Schlagwörter: traumatic brain injury
decompressive craniectomy
brain edema
hypothermia
aquaporin-4
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Both hypothermia and decompressive craniectomy have been considered as a treatment for traumatic brain injury. In previous experiments we established a murine model of decompressive craniectomy and we presented attenuated edema formation due to focal brain cooling. Since edema development is regulated via function of water channel proteins, our hypothesis was that the effects of decompressive craniectomy and of hypothermia are associated with a change in aquaporin-4 (AQP4) concentration. Male CD-1 mice were assigned into following groups (n = 5): sham, decompressive craniectomy, trauma, trauma followed by decompressive craniectomy and trauma + decompressive craniectomy followed by focal hypothermia. After 24 h, magnetic resonance imaging with volumetric evaluation of edema and contusion were performed, followed by ELISA analysis of AQP4 concentration in brain homogenates. Additional histopathological analysis of AQP4 immunoreactivity has been performed at more remote time point of 28d. Correlation analysis revealed a relationship between AQP4 level and both volume of edema (r 2 = 0.45, p < 0.01, ∗∗) and contusion (r 2 = 0.41, p < 0.01, ∗∗) 24 h after injury. Aggregated analysis of AQP4 level (mean ± SEM) presented increased AQP4 concentration in animals subjected to trauma and decompressive craniectomy (52.1 ± 5.2 pg/mL, p = 0.01; ∗ ), but not to trauma, decompressive craniectomy and hypothermia (45.3 ± 3.6 pg/mL, p > 0.05; ns) as compared with animals subjected to decompressive craniectomy only (32.8 ± 2.4 pg/mL). However, semiquantitative histopathological analysis at remote time point revealed no significant difference in AQP4 immunoreactivity across the experimental groups. This suggests that AQP4 is involved in early stages of brain edema formation after surgical decompression. The protective effect of selective brain cooling may be related to change in AQP4 response after decompressive craniectomy. The therapeutic potential of this interaction should be further explored.
DOI der Erstveröffentlichung: 10.3389/fneur.2018.00799
URL der Erstveröffentlichung: https://www.frontiersin.org/articles/10.3389/fneur.2018.00799
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-401642
hdl:20.500.11880/36139
http://dx.doi.org/10.22028/D291-40164
ISSN: 1664-2295
Datum des Eintrags: 20-Jul-2023
Bezeichnung des in Beziehung stehenden Objekts: Supplementary Material
In Beziehung stehendes Objekt: https://www.frontiersin.org/articles/file/downloadfile/400932_supplementary-materials_datasheets_1_docx/octet-stream/Data%20Sheet%201.docx/3/400932
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Neurochirurgie
M - Radiologie
Professur: M - Prof. Dr. Arno Bücker
M - Prof. Dr. Joachim Oertel
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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