Bitte benutzen Sie diese Referenz, um auf diese Ressource zu verweisen: doi:10.22028/D291-40057
Dateien zu diesem Datensatz:
Datei Beschreibung GrößeFormat 
fpls-10-01264.pdf4,98 MBAdobe PDFÖffnen/Anzeigen
Titel: A Novel Prokaryote-Type ECF/ABC Transporter Module in Chloroplast Metal Homeostasis
VerfasserIn: Voith von Voithenberg, Lena
Park, Jiyoung
Stübe, Roland
Lux, Christopher
Lee, Youngsook
Philippar, Katrin UdsID
Sprache: Englisch
In:
Titel: Frontiers in Plant Science
Bandnummer: 10
Verlag/Plattform: Frontiers
Erscheinungsjahr: 2019
Freie Schlagwörter: ABC transporter
chloroplast
energy-coupling factor transporter
inner envelope membrane
iron transport
metal homeostasis
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: During evolution, chloroplasts, which originated by endosymbiosis of a prokaryotic ancestor of today’s cyanobacteria with a eukaryotic host cell, were established as the site for photosynthesis. Therefore, chloroplast organelles are loaded with transition metals including iron, copper, and manganese, which are essential for photosynthetic electron transport due to their redox capacity. Although transport, storage, and cofactor-assembly of metal ions in chloroplasts are tightly controlled and crucial throughout plant growth and development, knowledge on the molecular nature of chloroplast metal-transport proteins is still fragmentary. Here, we characterized the soluble, ATP-binding ABCtransporter subunits ABCI10 and ABCI11 in Arabidopsis thaliana, which show similarities to components of prokaryotic, multisubunit ABC transporters. Both ABCI10 and ABCI11 proteins appear to be strongly attached to chloroplast-intrinsic membranes, most likely inner envelopes for ABCI10 and possibly plastoglobuli for ABCI11. Loss of ABCI10 and ABCI11 gene products in Arabidopsis leads to extremely dwarfed, albino plants showing impaired chloroplast biogenesis and deregulated metal homeostasis. Further, we identified the membrane-intrinsic protein ABCI12 as potential interaction partner for ABCI10 in the inner envelope. Our results suggest that ABCI12 inserts into the chloroplast inner envelope membrane most likely with five predicted α-helical transmembrane domains and represents the membrane-intrinsic subunit of a prokaryotic-type, energy-coupling factor (ECF) ABC-transporter complex. In bacteria, these multisubunit ECF importers are widely distributed for the uptake of nickel and cobalt metal ions as well as for import of vitamins and several other metabolites. Therefore, we propose that ABCI10 (as the ATPase A-subunit) and ABCI12 (as the membrane-intrinsic, energy-coupling T-subunit) are part of a novel, chloroplast envelope-localized, AAT energy-coupling module of a prokaryotic-type ECF transporter, most likely involved in metal ion uptake.
DOI der Erstveröffentlichung: 10.3389/fpls.2019.01264
URL der Erstveröffentlichung: https://www.frontiersin.org/articles/10.3389/fpls.2019.01264
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-400575
hdl:20.500.11880/36066
http://dx.doi.org/10.22028/D291-40057
ISSN: 1664-462X
Datum des Eintrags: 3-Jul-2023
Bezeichnung des in Beziehung stehenden Objekts: Supplementary Material
In Beziehung stehendes Objekt: https://www.frontiersin.org/articles/file/downloadfile/454248_supplementary-materials_images_11_pdf/octet-stream/Image%2011.pdf/1/454248
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Prof. Dr. Katrin Philippar
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes



Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons