Please use this identifier to cite or link to this item: doi:10.22028/D291-39941
Title: Effect of Cell Age and Membrane Rigidity on Red Blood Cell Shape in Capillary Flow
Author(s): Nouaman, Mohammed
Darras, Alexis
John, Thomas
Simionato, Greta
Rab, Minke A. E.
van Wijk, Richard
Laschke, Matthias W.
Kaestner, Lars
Wagner, Christian
Recktenwald, Steffen M.
Language: English
Title: Cells
Volume: 12
Issue: 11
Publisher/Platform: MDPI
Year of Publication: 2023
Free key words: red blood cells
membrane rigidity
cell shape
density separation
red blood cell senescence
DDC notations: 500 Science
610 Medicine and health
Publikation type: Journal Article
Abstract: Blood flow in the microcirculatory system is crucially affected by intrinsic red blood cell (RBC) properties, such as their deformability. In the smallest vessels of this network, RBCs adapt their shapes to the flow conditions. Although it is known that the age of RBCs modifies their physical properties, such as increased cytosol viscosity and altered viscoelastic membrane properties, the evolution of their shape-adapting abilities during senescence remains unclear. In this study, we investigated the effect of RBC properties on the microcapillary in vitro flow behavior and their characteristic shapes in microfluidic channels. For this, we fractioned RBCs from healthy donors according to their age. Moreover, the membranes of fresh RBCs were chemically rigidified using diamide to study the effect of isolated graded-membrane rigidity. Our results show that a fraction of stable, asymmetric, off-centered slipper-like cells at high velocities decreases with increasing age or diamide concentration. However, while old cells form an enhanced number of stable symmetric croissants at the channel centerline, this shape class is suppressed for purely rigidified cells with diamide. Our study provides further knowledge about the distinct effects of age-related changes of intrinsic cell properties on the single-cell flow behavior of RBCs in confined flows due to inter-cellular age-related cell heterogeneity.
DOI of the first publication: 10.3390/cells12111529
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-399415
ISSN: 2073-4409
Date of registration: 12-Jun-2023
Description of the related object: Supplementary Materials
Related object:
Faculty: M - Medizinische Fakultät
NT - Naturwissenschaftlich- Technische Fakultät
Department: M - Chirurgie
NT - Physik
Professorship: M - Prof. Dr. Michael D. Menger
M - Keiner Professur zugeordnet
NT - Prof. Dr. Christian Wagner
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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