Please use this identifier to cite or link to this item: doi:10.22028/D291-39938
Title: Immunohistochemistry Reveals TRPC Channels in the Human Hearing Organ : A Novel CT-Guided Approach to the Cochlea
Author(s): Englisch, Colya N.
Steinhäuser, Jakob
Wemmert, Silke
Jung, Martin
Gawlitza, Joshua
Wenzel, Gentiana
Schick, Bernhard
Tschernig, Thomas
Language: English
Title: International Journal of Molecular Sciences
Volume: 24
Issue: 11
Publisher/Platform: MDPI
Year of Publication: 2023
Free key words: TRPC6
human cochlea
organ of Corti
temporal bone
body donors
computed tomography
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: TRPC channels are critical players in cochlear hair cells and sensory neurons, as demonstrated in animal experiments. However, evidence for TRPC expression in the human cochlea is still lacking. This reflects the logistic and practical difficulties in obtaining human cochleae. The purpose of this study was to detect TRPC6, TRPC5 and TRPC3 in the human cochlea. Temporal bone pairs were excised from ten body donors, and the inner ear was first assessed based on computed tomography scans. Decalcification was then performed using 20% EDTA solutions. Immunohistochemistry with knockout-tested antibodies followed. The organ of Corti, the stria vascularis, the spiral lamina, spiral ganglion neurons and cochlear nerves were specifically stained. This unique report of TRPC channels in the human cochlea supports the hypothesis of the potentially critical role of TRPC channels in human cochlear health and disease which has been suggested in previous rodent experiments.
DOI of the first publication: 10.3390/ijms24119290
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-399384
ISSN: 1422-0067
Date of registration: 12-Jun-2023
Faculty: M - Medizinische Fakultät
Department: M - Anatomie und Zellbiologie
M - Hals-Nasen-Ohrenheilkunde
M - Medizinische Biochemie und Molekularbiologie
Professorship: M - Prof. Dr. Carola Meier
M - Prof. Dr. Bernhard Schick
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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